Expression dynamics and antiviral role of chicken OASL during infectious bronchitis virus infection.

Yang Chen, Qiqi Hu, Zongyi Bo, Mengjiao Guo, Chengcheng Zhang, Yongzhong Cao, Yantao Wu, Xiaorong Zhang

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Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Yang ChenJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.

Qiqi HuJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.

Zongyi BoThe International Joint Research Laboratory of Agricultural and Agri-product Safety, Yangzhou University, Yanzhou, Jiangsu 225009, China.

Mengjiao GuoJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.

Chengcheng ZhangJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.

Yongzhong CaoThe International Joint Research Laboratory of Agricultural and Agri-product Safety, Yangzhou University, Yanzhou, Jiangsu 225009, China.

Yantao WuJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; The International Joint Research Laboratory of Agricultural and Agri-product Safety, Yangzhou University, Yanzhou, Jiangsu 225009, China. Electronic address: ytwu@yzu.edu.cn.

Xiaorong ZhangJiangsu Co-Innovation Center for the Prevention and Control of Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. Electronic address: zxr@yzu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

OASL, a distinctive member of the 2'-5'-oligoadenylate synthetase (OAS) family, is a well-characterized interferon-stimulated gene (ISG). In mammals and certain avian species, OASL exhibits dual functions in antiviral defense and innate immune regulation. However, the biological role of chicken OASL (chOASL) remains poorly understood, largely due to the natural absence of RIG-I in chickens. Infectious bronchitis virus (IBV) belongs to Gammacoronavirus genus and causes substantial economic losses in the global poultry industry. To explore whether chOASL is involved in the host defense against IBV, this study aimed to comprehensively characterize its expression dynamics and antiviral function. Firstly, the anti-chOASL polyclonal antibody was generated using prokaryotically expressed recombinant protein. Using this validated tool, we demonstrated that endogenous chOASL was significantly induced by Poly(I:C) and QX-IBV in various chicken cells. Furthermore, we mapped the baseline tissue distribution of chOASL protein for the first time and proved that chOASL was markedly upregulated, particularly in immune organs and IBV target tissues during IBV infection. Importantly, overexpression and knockdown assays verified that chOASL could suppress IBV replication. In conclusion, this study not only provides a reliable tool for detecting chOASL but also establishes its specific expression profile and direct antiviral activity against IBV, laying a solid foundation for further elucidating antiviral mechanisms of chOASL.

Indexed as

2',5'-Oligoadenylate SynthetaseAvian ProteinsChickensCoronavirus InfectionsImmunity, InnateInfectious bronchitis virusPoultry DiseasesAnimals2',5'-Oligoadenylate SynthetaseAvian ProteinsAntiviral activityChicken OASLIBVPolyclonal antibody

Identifiers

PMID42102758

DOI10.1016/j.psj.2026.107014

PMCPMC13185871

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LicenceCC BY-NC-ND

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