ArticlePoultry science2026
Hormetic dose-response of pure hydroxytyrosol in laying hens: Optimal dose for egg shelf-life extension and yolk docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid concentration.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydroxytyrosol (HT) is a potent olive-derived antioxidant, but its dose-response effects on laying hen performance, gut health, and egg quality remain poorly defined. This study evaluated the effects of dietary supplementation with pure HT (0, 5, 10, 20, and 50 mg/kg) on productive performance, intestinal morphometry, oxidative stability, and egg quality in ISA Brown hens at 80 weeks of age. Performance traits and serum biochemical indicators were not influenced by HT supplementation (P > 0.05). Regarding intestinal morphometry, villus height decreased linearly with increasing HT (P < 0.001), suggesting a physiological threshold at higher doses. For egg quality, HT reduced yolk TBARS linearly after 30 days at 22 ± 4°C (P < 0.001), while Haugh units showed a quadratic, hormetic response with best preservation at 10-20 mg/kg (P < 0.001) and lower efficacy at 50 mg/kg. Docosahexaenoic acid (DHA) content in yolk was highest at 20 mg/kg (P = 0.032), with a quadratic response for eicosapentaenoic acid (EPA) concentration (Quadratic effect: P = 0.016). Multivariate analysis (PCA/HCPC) confirmed distinct metabolic profiles and identified 20 mg/kg as the optimal biological threshold. In conclusion, 20 mg/kg pure HT is the optimal dose to extend egg shelf life and enrich eggs with n-3 PUFAs, whereas 50 mg/kg exceeds the physiological threshold, limiting intestinal morphology and egg quality. These results provide a clear dose recommendation for producing functional eggs with enhanced oxidative stability and DHA content under non-refrigerated storage and are particularly relevant for egg production systems in tropical and subtropical regions where non-refrigerated storage is common.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.