Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM.

Uwe Pfeil, Felix Dettler, Alexander Perniss, Praju Vikas Anekal, Lora Bankova, Paula Montero LIopis, Klaus Deckmann

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Uwe PfeilInstitute for Anatomy and Cell Biology, Justus-Liebig-University, Giessen, Germany.

Felix DettlerInstitute for Anatomy and Cell Biology, Justus-Liebig-University, Giessen, Germany.

Alexander PernissDivision of Allergy and Clinical Immunology, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, USA.

Praju Vikas AnekalMicRoN Core, Harvard Medical School, Boston, MA, USA.

Lora BankovaDivision of Allergy and Clinical Immunology, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, USA.

Paula Montero LIopisMicRoN Core, Harvard Medical School, Boston, MA, USA.

Klaus DeckmannInstitute for Anatomy and Cell Biology, Justus-Liebig-University, Giessen, Germany. Electronic address: klaus.deckmann@anatomie.med.uni-giessen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The distribution of cells within organs, their involvement in tissue structure, and their organization in three-dimensional networks are important aspects in the analysis and understanding of biological processes. Here, we present a protocol for preparing whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by confocal laser scanning microscopy. We describe steps for perfusion, preparation, tissue clearing, and analysis using confocal laser scanning microscopy. We then detail procedures for three-dimensional processing of the murine urethra and urinary bladder. For complete details on the use and execution of this protocol, please refer to Schmidt et al.

Indexed as

ImmunohistochemistryUrethraUrinary BladderAnimalsFemaleMiceMicroscopy, ConfocalPerfusionCell BiologyMicroscopy

Identifiers

PMID42101941

DOI10.1016/j.xpro.2026.104542

PMCPMC13186176

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.