ArticleCellular oncology (Dordrecht, Netherlands)2026
The ubiquitin-like protein FAT10 enhances the autophagy-mediated degradation of ZO-1 by stabilizing ATG3 to promote the lung metastasis of colon cancer.
Article in Cellular oncology (Dordrecht, Netherlands), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
purposeThe ubiquitin-like modifier HLA-F adjacent transcript 10 (FAT10) directs substrates to the 26 S proteasome, but its role in autophagic protein degradation remains unclear.
methodsFAT10 expression was analyzed by qRT-PCR and western blotting in colon cancer (CC) tissues and cells. Bioinformatics revealed FAT10-associated biological processes in CC. In vitro and in vivo assays examined CC cell invasion and metastasis. Autophagy was assessed by western blotting, mRFP-GFP-LC3 reporter (tfLC3), and electron microscopy. In vitro ubiquitination assays measured ubiquitination of zona occludens 1 (ZO-1) and autophagy-related gene 3 (ATG3). liquid chromatography‒tandem mass spectrometry (LC-MS/MS) identified FAT10-interacting proteins. Co-IP and GST pull-down confirmed FAT10-ATG3 binding.
resultsFAT10 was upregulated in CC tissues and cells. Bioinformatic analyses revealed that FAT10 expression was correlated with extracellular matrix binding and cell migration in CC. FAT10 overexpression enhanced CC invasion and metastasis. FAT10 promoted autophagic degradation of ZO-1, a key component of tight junctions, facilitating invasion and migration. Mechanistically, FAT10 stabilized ATG3 by competing with ubiquitin for binding, inhibiting ATG3 ubiquitination and activating autophagy, leading to ZO-1 degradation. The ATG3 inhibitor compound 189 reversed FAT10-induced ZO-1 loss and suppressed lung metastasis.
conclusionOur findings demonstrate a novel pathway by which FAT10 activates autophagy by stabilizing ATG3, leading to ZO-1 degradation and promoting CC lung metastasis. This study provides new insights into the role of FAT10 in regulating protein homeostasis and offers a potential strategy for targeting the FAT10-ATG3-autophagy axis in the treatment of CC metastasis.
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