Evidence map›Paper›PMID 42101705›Full record

ArticleWorld journal of microbiology & biotechnology2026

Inhibitory effects of esculetin as a quorum sensing inhibitor on biofilm formation and virulence factors in Vibrio anguillarum.

Qinghua Yu, Xiaomin Xie, Hongming Tang, Yuqian Wang, Yunpeng Wu, Ruogu Zhang, Qi Gao, Zhixin Guo, Shigen Ye, Han Zhou and 1 more

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Qinghua Yu *Aquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Xiaomin Xie *Ganjiang Chinese Medicine Innovation Center, Nanchang, 330000, China.
Hongming TangCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Yuqian WangAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Yunpeng WuAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Ruogu ZhangAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Qi GaoAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Zhixin GuoAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Shigen YeAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China.
Han ZhouCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. zhouhan418@dicp.ac.cn.
Weijia ZhouAquatic Animal Hospital, Dalian Ocean University, Dalian, 116023, China. zhouweijia@dlou.edu.cn.

Funding

2025 China Undergraduate Innovation and Entrepreneurship Training Program S202510158020Liaoning Provincial Science and Technology Joint Program 2025-MSLH-134Special Fund for DigitalEconomy on Budgetary Infrastructure Investment of Jiangxi Province 2506-360000-04-04-511042
6 · The paper itself

Abstract

Vibrio anguillarum (V. anguillarum) causes vibriosis in aquaculture, with pathogenicity regulated by quorum sensing (QS). Inhibiting QS is a promising anti-virulence strategy. Plant-derived compounds are attractive due to their safety and low resistance potential. Here, we screened 33 natural compounds and found that esculetin, a coumarin, inhibited V. anguillarum with a minimum inhibitory concentration (MIC) of 40 mg/L. At sub-inhibitory concentrations, esculetin effectively reduced biofilm formation and its key component extracellular polymeric substances (EPS) by 45.84% and 27.23%, respectively. Additionally, esculetin reduced bacterial swarming and swimming motility diameters by 6.67% and 25.76%, respectively, while partially suppressing extracellular protease and hemolytic activities. RT-qPCR (quantitative real-time PCR) analyses revealed that esculetin downregulated genes associated with the QS system (vanR, rpoN, vanT, and vanO) and virulence phenotypes (ompU, flaB, and hlyU). Molecular docking and dynamics simulations indicated that esculetin interacts with VanR and VanT proteins, with a stronger binding stability observed for VanT. In conclusion, esculetin acts as an effective QS inhibitor and shows potential as a novel candidate drug for controlling V. anguillarum infections, offering a new approach for the prevention and treatment of aquatic vibriosis.

Indexed as

Anti-Bacterial AgentsBiofilmsQuorum SensingUmbelliferonesVibrioVirulence FactorsAnimalsBacterial ProteinsGene Expression Regulation, BacterialMicrobial Sensitivity TestsMolecular Docking SimulationVirulenceAnti-Bacterial AgentsBacterial ProteinsesculetinUmbelliferonesVirulence FactorsAquacultureBiofilmEsculetinQuorum sensingVibrio anguillarum

Identifiers

PMID42101705

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.