Evidence map›Paper›PMID 42099561›Full record

ArticleBiosafety and health2026

Development of monoclonal antibody-based methods for detecting mpox virus.

Zuoyuan Du, Xingqi Wu, Li Guo, Qiao Zhang, He Huang, Lili Ren, Jianwei Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosafety and health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zuoyuan DuNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.
Xingqi WuNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.
Li GuoNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.
Qiao ZhangNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.
He HuangNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.
Lili RenNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.
Jianwei WangNational Health Commission Key Laboratory of Systems Biology of Pathogens, State Key Laboratory of Respiratory Health and Multimorbidity, Christophe Mérieux Laboratory, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102600, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The World Health Organization declared mpox a public health emergency of international concern in both 2022 and 2024, highlighting the critical need for rapid and reliable diagnostic solutions. To address this challenge, we developed monoclonal antibodies against four mpox virus (MPXV) antigens (A29L, A35R, H3L, and E8L) using hybridoma technology. Epitope binning analyses, performed using competitive enzyme-linked immunosorbent assay (ELISA) and biolayer interferometry, identified non-overlapping antibody pairs (e.g., 18F7-27C3 for A29L and 4E7-6C11 for A35R), which served as the foundation for sandwich ELISA assays exhibiting nanogram-level sensitivity. These antibody pairs demonstrated high specificity, effectively distinguishing MPXV antigens from homologous proteins of cowpox virus, vaccinia virus, and variola virus, while maintaining reactivity toward cultured MPXV. Collectively, this work establishes a robust immunodiagnostic platform with strong translational potential for point-of-care applications during mpox outbreaks.

Indexed as

Antigen detectionHybridoma technologyMonoclonal antibodies (mAb)Mpox virus (MPXV)

Identifiers

PMID42099561
PMCPMC13147770

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.