ArticleBiosafety and health2026
A rapid and sensitive yellow fever virus detection method based on CRISPR/Cas13a and reverse transcription recombinase-aided amplification with special lateral-flow test strips.
Article in Biosafety and health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Yellow fever (YF) is an epidemic disease caused by the yellow fever virus (YFV). Historically, it has caused several epidemics and continues to result in fatalities in South Sudan and other regions today. Due to its limited therapeutic options, high mortality rate, and high transmissibility, YFV is classified as a biosafety level-3 (BSL-3) pathogen. The development of a rapid and sensitive YFV detection method is therefore important for epidemic prevention and response. Herein, we combined the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) 13a system, reverse transcription recombinase-aided amplification (RT-RAA), and the easy-readout, sensitive enhanced lateral flow strip (ERASE LFS) to establish a new detection method for YFV. YFV ribonucleic acid and infectious YFV 17D particles were effectively detected using this approach. The RT-RAA-CRISPR-ERASE LFS (RCE) assay for YFV RNA demonstrated a detection sensitivity of 10
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