ArticleCureus2026
Rapid Detection of Hypervirulent Klebsiella pneumoniae by Multiplex PCR and Nucleic Acid Chromatography: Evaluation of Diagnostic Performance and Clinical Utility.
Article in Cureus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 authors.
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Abstract
BACKGROUND AND
aimHypervirulent
methodsWe established a multiplex PCR and nucleic acid chromatography system capable of detecting three virulence genes (prmpA, iucA-PP2, and peg-344-PP2). We included 119 cases of bacteremia caused by isolates identified as
resultsIsolates that were string test-negative but carried hvKp-associated virulence genes were identified, suggesting that reliance on phenotypic methods alone may lead to underdiagnosis of hypervirulent strains. In a receiver operating characteristic curve analysis, positivity for three or more markers among the four variables (string test and the three virulence-associated genes) yielded a sensitivity of 0.84 and specificity of 0.90. High concordance rates were also observed between singleplex and multiplex PCR using either gel electrophoresis or DNA chromatography.
conclusionsHvKp-associated genes can be detected more rapidly by combining multiplex PCR with nucleic acid chromatography than by conventional gel electrophoresis-based methods. This approach also demonstrated high sensitivity and may be helpful for potential clinical risk assessment of hvKp infections.
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