Evidence map›Paper›PMID 42098883›Full record

ArticlePlant, cell & environment2026

PuCBL9-PuCIPK11 Modulates Stomatal Microfilament Reorganisation in a Phosphorylation-Dependent Manner to Enhance Drought Tolerance in Poplar.

Jingjing Li, Lu Yang, Qianqian Liu, Sophia Hydarry Matola, Xinyu Cui, Haoqin Zhao, Wanxin Li, Dativa Gosbert Tibesigwa, Tabeer Gulfam, Jingli Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant, cell & environment, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jingjing LiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Lu YangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Qianqian LiuState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Sophia Hydarry MatolaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Xinyu CuiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Haoqin ZhaoState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Wanxin LiState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Dativa Gosbert TibesigwaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Tabeer GulfamState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Jingli YangState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.ORCID https://orcid.org/0000-0003-0922-5954

Funding

Fundamental Research Funds for the Central Universities 2572025AW27National Natural Science Foundation of China 31870649
6 · The paper itself

Abstract

Drought stress severely constrains the growth and survival of forest trees. Plant stomata serve as portals for water transpiration and gas exchange, where adjustments in stomatal aperture critically influence drought resistance in trees. Although guard cell actin cytoskeleton dynamics are essential for stomatal movement, their regulation in trees remains poorly understood. This study characterises PuCIPK11 in Populus ussuriensis Kom., demonstrating its role in enhancing drought resistance via stomatal closure. PuCIPK11 predominantly expressed in guard cells and induced by drought and ABA, and its overexpression promotes microfilaments (MFs) depolymerisation and longitudinal rearrangement, accelerating stomatal closure. Under drought stress, PuCIPK11 is recruited to the plasma membrane by the Ca²⁺ sensor PuCBL9, where it undergoes conformational activation through the formation of a PuCBL9-PuCIPK11 module. Furthermore, PuCIPK11 phosphorylates PuCBL9 at Ser201, a process enhanced by ABA that strengthens their interaction. Functional validation using pucbl9 knockout mutants in a PuCIPK11-overexpression background confirmed that PuCIPK11 acts in concert with PuCBL9 to modulate MFs dynamics and drought tolerance. This study provides mechanistic insights into the phosphorylation-dependent regulation of stomatal cytoskeletal dynamics and identifies the PuCBL9-PuCIPK11 module as a key regulator of drought resistance in poplar, offering potential targets for genetic improvement of poplar.

Indexed as

Actin CytoskeletonPlant ProteinsPlant StomataPopulusAbscisic AcidDrought ResistanceDroughtsGene Expression Regulation, PlantPhosphorylationPlants, Genetically ModifiedStress, PhysiologicalAbscisic AcidPlant ProteinsCBL9‐CIPK11droughtmicrofilamentsphosphorylationPopulus ussuriensis Komstomatal movement

Identifiers

PMID42098883
PMCPMC13353596

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.