ArticleBMC veterinary research2026
Cryoprotective effects of cholesterol-loaded cyclodextrin and iodixanol on ram sperm: assessment of post-thaw quality and in vitro fertilization outcomes.
Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
backgroundThis study aimed to investigate the effects of cholesterol-loaded cyclodextrin (CLC) and iodixanol, added individually or in combination to a ram semen extender, on post-thaw sperm quality, in vitro fertilization outcomes, and embryo development. Semen samples were divided into four experimental groups: Control, CLC (2 mg CLC/120 × 10
resultsCLC and CLC + Iodixanol significantly preserved total motility (%), acrosomal integrity (%), viability (%), and plasma membrane functionality (%) after thawing (p < 0.05). Iodixanol alone did not provide significant protection, particularly regarding membrane integrity (p > 0.05). Although both additives showed potential in supporting in vitro embryonic development (p < 0.05), their combined use did not result in a synergistic effect (p > 0.05).
conclusionCholesterol-loaded cyclodextrin supplementation effectively enhanced post-thaw sperm quality parameters and supported in vitro embryonic development, whereas iodixanol provided limited cryoprotective benefits; however, their combination did not yield synergistic effects, indicating that further in vivo investigations are warranted to establish their practical utility in ovine reproductive biotechnology.
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