Combination of magnetic bead extraction and LC-MS/MS detection for the detection of saliva cortisol.

Liping Zhu, Tingjun Chen, JiaYing Ni, Jingyi Lu, Wei Lu, Wenshuo Han, Qian Dou, Jian Zhou

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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8 authors.

Liping ZhuHenan Institute of Advanced Technology, Zhengzhou University, Zhengzhou, 450003, China.

Tingjun ChenLaboratory of Nanophotonic Materials and Devices, Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, 100190, People's Republic of China.

JiaYing NiDepartment of Endocrinology and Metabolism, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, China.

Jingyi LuDepartment of Endocrinology and Metabolism, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, China.

Wei LuDepartment of Endocrinology and Metabolism, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, China.

Wenshuo HanDepartment of Endocrinology and Metabolism, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, China.

Qian DouSchool of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China. douq@nanoctr.cn.

Jian ZhouDepartment of Endocrinology and Metabolism, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Clinical Center for Diabetes, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai, 200233, China. zhoujian@sjtu.edu.cn.

Funding

National Natural Science Foundation of China 52472156National Natural Science Foundation of China L2324121

6 · The paper itself

Abstract

Salivary cortisol is an important biomarker for the assessment of stress-related disorders and endocrine function. Due to the noninvasive and convenient nature of saliva sampling, it has considerable potential for clinical application. However, the low concentration of cortisol in saliva, typically at the nanograms-per-milliliter level, together with the complexity of the salivary matrix, poses substantial challenges to analytical sensitivity and selectivity. In this study, an analytical method for salivary cortisol determination was developed based on immunomagnetic bead enrichment and purification coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Cortisol monoclonal antibodies were conjugated to carboxyl-modified nanomagnetic beads, and the target analyte was extracted and purified through immunoaffinity adsorption followed by ethanol elution. The method exhibited good linearity over the ranges of 0.10-1.00 ng/mL and 1.00-20.00 ng/mL, with a limit of detection (LOD) of 0.04 ng/mL and a lower limit of quantification (LLOQ) of 0.10 ng/mL. The method also showed satisfactory accuracy, with spiked recoveries ranging from 80% to 110%. In addition, salivary cortisol concentrations showed a strong positive correlation with serum cortisol concentrations (Pearson correlation coefficient, r = 0.91636). These results indicate that the proposed method provides a sensitive, selective, and reliable approach for salivary cortisol analysis and shows promise for clinical application.

Indexed as

HydrocortisoneImmunomagnetic SeparationLiquid Chromatography-Mass SpectrometrySalivaTandem Mass SpectrometryChromatography, LiquidHumansLimit of DetectionHydrocortisoneImmunomagnetic beadLiquid chromatography-tandem mass spectrometrySalivary cortisolSample preparation

Identifiers

PMID42098506

DOI10.1016/j.slast.2022.01.006

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