Evidence map›Paper›PMID 42097140›Full record

ArticleStructure (London, England : 1993)2026

Tracking the redox reaction of the iron enzyme ribonucleotide reductase using continuous SerialED and SFX.

Laura Pacoste, Rohit Kumar, Vivek Srinivas, Hiroki Makita, Philipp S Simon, Rahul Bannerjee, Natalie M Minnetian, Asmit Bhowmick, Daniel W Paley, David W Mittan-Moreau and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Laura PacosteDepartment of Chemistry, Stockholm University, Stockholm, Sweden.
Rohit KumarDepartment of Biochemistry and Biophysics and Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
Vivek SrinivasDepartment of Biochemistry and Biophysics and Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
Hiroki MakitaMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Philipp S SimonMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Rahul BannerjeeDepartment of Chemistry and Biochemistry, Auburn University, Auburn, AL 36849, USA.
Natalie M MinnetianMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Asmit BhowmickMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Daniel W PaleyMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
David W Mittan-MoreauMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Kuntal ChatterjeeMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Daniel J RosenbergLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Alexander BatyukLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Leland B GeeLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Roberto Alonso-MoriLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Nicholas K SauterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Junko YanoMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Vittal K YachandraMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Juliane JohnDepartment of Biochemistry and Biophysics and Science for Life Laboratory, Stockholm University, Stockholm, Sweden; Technical University Dortmund, Faculty for Chemistry and Chemical Biology, Dortmund, Germany.
Oskar AureliusMAX IV Laboratory, Lund, Sweden.
Aaron S BrewsterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Jan F KernMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Buster BlombergDepartment of Chemistry, Stockholm University, Stockholm, Sweden.
Hugo LebretteDepartment of Biochemistry and Biophysics and Science for Life Laboratory, Stockholm University, Stockholm, Sweden; Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, CNRS, Université de Toulouse, Toulouse, France.
Hongyi XuDepartment of Chemistry, Stockholm University, Stockholm, Sweden.
Gerhard HoferDepartment of Chemistry, Stockholm University, Stockholm, Sweden.
Martin HögbomDepartment of Biochemistry and Biophysics and Science for Life Laboratory, Stockholm University, Stockholm, Sweden. Electronic address: martin.hogbom@dbb.su.se.
Xiaodong ZouDepartment of Chemistry, Stockholm University, Stockholm, Sweden. Electronic address: xiaodong.zou@su.se.

Funding

Structural Dynamics at LCLSP41GM139687 · NIGMS · STANFORD UNIVERSITY · PI HUNTER, MARK · 2021 to 2025
$7.5M
Biological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8M
High throughput sample delivery method for time resolved studies of enzyme reactions with X-ray and complementary techniquesR01GM126289 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI KERN, JAN F · 2017 to 2025
$4.6M
DIALS: supporting structural biology through open source diffraction processing softwareR24GM154040 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Aaron Samuel Brewster · 2024 to 2026
$3.7M
Cctbx.xfel: Serial crystallography computational methods aimed at biomolecular functionR35GM151988 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI NICHOLAS K SAUTER · 2024 to 2026
$2.2M
High-end X-ray Detector System for Femtosecond CrystallographyS10OD023453 · OD · STANFORD UNIVERSITY · PI BOUTET, SEBASTIEN · 2017 to 2017
$1.7M
NIGMS NIH HHS P41 GM139687NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R01 GM126289NIGMS NIH HHS R24 GM154040NIGMS NIH HHS R35 GM151988NIH HHS S10 OD023453
6 · The paper itself

Abstract

Serial femtosecond crystallography (SFX) and continuous serial electron diffraction (c-SerialED) both enable high-resolution structure determination from protein microcrystals with minimal radiation damage, making it ideal for studying redox-active metalloenzymes. Here, c-SerialED and SFX were used to solve structures of the class Ia ribonucleotide reductase R2 subunit in oxidized (Fe

Indexed as

IronRibonucleotide ReductasesCrystallography, X-RayModels, MolecularOxidation-ReductionProtein ConformationIronribonucleotide reductase R2 subunitRibonucleotide Reductaseselectrostatic potential mapsmetalloenzymesmicrocrystalsoxidation stateradiation damageribonucleotide reductaseserial electron diffractionserial femtosecond crystallographytime-resolved crystallography

Identifiers

PMID42097140
PMCPMC13166098

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.