Evidence map›Paper›PMID 42096887›Full record

ReviewCurrent opinion in structural biology2026

Three-color single-molecule fluorescence resonance energy transfer to study macromolecular dynamics.

Jia Gao, Bo Yuan, Sourav Mondal, Rajib Basak, Tae-Hee Lee

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Current opinion in structural biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jia GaoDepartment of Chemistry, The Pennsylvania State University, University Park, 16802 PA, USA.
Bo YuanDepartment of Chemistry, The Pennsylvania State University, University Park, 16802 PA, USA.
Sourav MondalDepartment of Chemistry, The Pennsylvania State University, University Park, 16802 PA, USA.
Rajib BasakDepartment of Chemistry, The Pennsylvania State University, University Park, 16802 PA, USA.
Tae-Hee LeeDepartment of Chemistry, The Pennsylvania State University, University Park, 16802 PA, USA. Electronic address: txl18@psu.edu.

Funding

Dynamics of DNA-histone interactionsR35GM148208 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI Tae-Hee Lee · 2023 to 2026
$2.5M
NIGMS NIH HHS R35 GM148208
6 · The paper itself

Abstract

Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful tool to probe macromolecular dynamics. Two-color smFRET, however, provides only one-dimensional distance information, limiting its use in complex systems. Multicolor smFRET with three or more fluorophores can overcome this, though three is often the practical limit due to the narrow selection of dyes with suitable spectral properties, the challenges of labeling with multiple fluorophores, and the complexity of data interpretation. Here, we survey recent investigations of the dynamics of macromolecules and their complexes based on three-color smFRET. In this review, three-color smFRET refers to the use of three fluorophores in a FRET system where two or all three fluorophores may participate in FRET at a given time point. We aim to highlight structural biology problems particularly well suited for three-color smFRET and to provide a brief overview of designing and implementing such experiments in light of their benefits and challenges.

Indexed as

Fluorescence Resonance Energy TransferMacromolecular SubstancesSingle Molecule ImagingColorFluorescent DyesFluorescent DyesMacromolecular Substances

Identifiers

PMID42096887
PMCPMC13524102

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.