An In Vitro Model for Studying Interactions Between Gastrointestinal Microbes and Planktonic and Sessile Clostridioides difficile Populations.

Jason Pizzini, Hugh C McCullough, Ben S Sidner, Robert A Britton, Kurt H Piepenbrink, Jennifer M Auchtung

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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5 · Who and what money

Authors and funding

6 authors.

Jason PizziniDepartment of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.

Hugh C McCulloughDepartment of Food Science and Technology and Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.

Ben S SidnerDepartment of Food Science and Technology and Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.

Robert A BrittonDepartment of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.

Kurt H PiepenbrinkDepartment of Food Science and Technology and Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA.

Jennifer M AuchtungDepartment of Food Science and Technology and Nebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA. jauchtung2@nebraska.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interactions between Clostridioides difficile, the gastrointestinal microbiota, and the host mucosal epithelium play important roles in governing the ability of C. difficile to colonize and cause disease. Several in vitro tools have been developed to investigate C. difficile physiology in the presence of microbial communities. In this chapter, we describe a model for studying C. difficile-mucin interactions in the presence of a complex microbiota using continuous flow bioreactors. This model can facilitate mechanistic studies of specific microbes and mucin structures important for C. difficile colonization, complementing findings from animal models. The approach presented here builds upon the preceding chapter's protocol for generating mucin hydrogels on glass slides and extends it to examine C. difficile adhesion to mucosal surfaces.

Indexed as

Clostridioides difficileGastrointestinal MicrobiomePlanktonAnimalsBacterial AdhesionBioreactorsHumansHydrogelsIntestinal MucosaModels, BiologicalMucinsHydrogelsMucinsColonization resistanceMicrobiotaMucin

Identifiers

PMID42096157

DOI10.1007/978-1-4939-6361-4_18

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.