Evidence map›Paper›PMID 42096151›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

An Integrated Workflow for the Purification and Fluorescence Imaging of Clostridioides difficile Spores Using Lanthanide Beta-Diketone Complexes.

Ajitha Sundaresan, Ian Cheong

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ajitha SundaresanTemasek Life Sciences Laboratory, Singapore, Singapore.
Ian CheongTemasek Life Sciences Laboratory, Singapore, Singapore. ian@tll.org.sg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a robust protocol for both the purification and imaging of C. difficile spores. First, we describe a simplified, one-step isopycnic centrifugation method using Percoll that enables efficient separation of spores from vegetative cells. Next, we introduce a fluorescence-based imaging method using lanthanide beta-diketone complexes (primarily Eu[TTA]

Indexed as

Clostridioides difficileKetonesLanthanoid Series ElementsOptical ImagingSpores, BacterialMicroscopy, ConfocalMicroscopy, FluorescenceStaining and LabelingWorkflowKetonesLanthanoid Series ElementsC. difficile sporesConfocal microscopyFluorescence microscopyLanthanide beta-diketonesLive imaging

Identifiers

PMID42096151

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.