Evidence map›Paper›PMID 42096150›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Preparation of Spores with the 70:30 Method.

Eliane de Oliveira Ferreira

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Eliane de Oliveira FerreiraLaboratory of Biology of Anaerobes, Medical Microbiology Department, Paulo de Goes Microbiology Institute, Federal University of Rio de Janeiro (UFRJ), Rio de Janiero, Brazil. eliane_ferreirarj@micro.ufrj.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clostridioides difficile spores are highly resistant, dormant structures that play a critical role in the transmission and pathogenesis of C. difficile infections. The purification of C. difficile spores is essential for understanding the mechanisms of spore formation, germination, and the disease. In this chapter, a method for the purification of C. difficile spores using a 70:30 medium is described, consisting of 70% SMC (sporulation medium for Clostridia) and 30% BHIS (brain-heart infusion supplement). The 70:30 medium is used to induce sporulation and produce high yields of pure C. difficile spores. A protocol for the preparation of the 70:30 medium, spore purification, and characterization of the purified spores is described. This method is a valuable tool for researchers studying the biology and pathogenesis of C. difficile and will facilitate the development of effective strategies for the prevention and treatment of C. difficile infections.

Indexed as

Clostridioides difficileSpores, BacterialCulture MediaCulture Media70:30 methodSpore inductionSpore purification

Identifiers

PMID42096150

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.