Evidence map›Paper›PMID 42096024›Full record

ArticleDigestive diseases and sciences2026

n-3 Polyunsaturated Fatty Acids Improve DSS-Induced Acute Colitis in Mice via Intestinal Barrier Fortification and Gut Microbiome Modulation.

Xinyi Hu, Jiahui Chen, Yiqiu Zhou, Xinyue Ji, Siqi Shen, Junhui Qian, Tao Li, Feng Xu, Ying Zhou, Dezheng Zhou and 1 more

Abstract read
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In one paragraph

Article in Digestive diseases and sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinyi Hu *School of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China.
Jiahui Chen *School of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China.
Yiqiu Zhou *The First Affiliated Hospital of Ningbo University, Ningbo, 315020, Zhejiang, China.
Xinyue JiSchool of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China.
Siqi ShenSchool of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China.
Junhui QianSchool of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China.
Tao LiHEALTH BioMed Research & Development Center, Health BioMed Co. Ltd., Ningbo, 315801, Zhejiang, China.
Feng XuDepartment of Gastroenterology, Li Huili Hospital Affiliated to Ningbo University, Ningbo, 315040, Zhejiang, China.
Ying ZhouDepartment of Gastroenterology, Li Huili Hospital Affiliated to Ningbo University, Ningbo, 315040, Zhejiang, China.
Dezheng ZhouSchool of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China. zhoudezheng@nbu.edu.cn.
Xiaohong ZhangSchool of Public Health, Health Science Center, Ningbo University, Zhejiang Key Laboratory of Pathophysiology, Ningbo, 315211, Zhejiang, China. zhangxiaohong1@nbu.edu.cn.

Funding

the National Natural Science Foundation of China 82273621the National Natural Science Foundation of China 82404257
6 · The paper itself

Abstract

backgroundThe increasing global burden of ulcerative colitis (UC) is showing a high incidence in developed countries and a swift rise in developing countries. n-3 polyunsaturated fatty acids (PUFAs), particularly eicosatetraenoic acid (EPA) and docosahexaenoic acid (DHA), have demonstrated anti-inflammatory effects. However, their exact mechanisms in intestinal barrier repair for UC remain incompletely elucidated.

methodsAcute UC was induced using 2% dextran sulfate sodium (DSS) and the therapeutic effects of mesalazine, EPA, DHA, and EPA + DHA were evaluated. The mucus barrier was assessed histologically. RT-qPCR and Western blot were used to quantify the expression of Mucin2, mechanical barrier proteins (Claudin-1/Occludin), and key signaling pathways (PI3K/Akt, TNF-α/NF-κB, GPR120/PKA/CREB). Gut microbiome composition was analyzed via 16S rRNA sequencing.

resultsEPA + DHA intervention demonstrated optimal efficacy in alleviating colitis, through establishing an anti-inflammatory colonic lipid microenvironment by elevating the EPA/AA and DHA/AA ratios. Inhibiting PI3K/Akt/NHE3, downregulating TNF-α/NF-κB/DRA, activating GPR120/PKA/CREB/AQP) improved the mucosal barrier and restored tight junction to enhance the mechanical barrier. Furthermore, EPA + DHA significantly increased the abundance of beneficial microbiome like Lachnospiraceae and Ruminococcaceae.

conclusionEPA + DHA effectively alleviates acute UC in mice by fortifying the colonic mucus-mechanical dual barrier and regulating gut microbiome homeostasis, providing a novel potential strategy for UC adjunctive treatment.

Indexed as

ColitisColitis, UlcerativeColonDocosahexaenoic AcidsEicosapentaenoic AcidGastrointestinal MicrobiomeIntestinal MucosaAnimalsDextran SulfateDisease Models, AnimalIntestinal Barrier FunctionMaleMiceMice, Inbred C57BLSignal TransductionDextran SulfateDocosahexaenoic AcidsEicosapentaenoic AcidGlycationGut microbiotaIon channel proteinn-3 polyunsaturated fatty acidsUlcerative colitis

Identifiers

PMID42096024

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.