ArticleRSC chemical biology2026
Methylene insertion reveals the importance of phosphate-phosphate distance in DNase I cleavage.
Article in RSC chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors.
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Abstract
DNase I is a major endonuclease in human serum that cleaves double-stranded DNA. While its activity has been characterized for sequence preference and structural requirements, the effects of chemical modifications on cleavage activity remain underexplored. Here, we report that the insertion of methylene groups at defined positions on DNA substrates significantly changes the DNase I cleavage in a position-dependent manner, leading to either enhancement or suppression depending on the insertion site. These findings indicated that chemical modifications can be used to modulate DNase I activity and suggested that local structure, particularly the distance between phosphate groups, had a significant impact on DNase I cleavage.
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