Evidence map›Paper›PMID 42094516›Full record

ArticlebioRxiv : the preprint server for biology2026

Stress Granule Sequestration of CCR4-NOT Promotes Poly(A) Lengthening of Stress-Survival Transcripts.

Yi-Cheng Tsai, Hiroyuki Uechi, Sean R Millar, Eileigh Kadijk, Alon Minkovich, John Paul Tsu Ouyang, Karl J Schreiber, Jie Qi Huang, Carina A Lyons, Vesal Kasmaeifar and 9 more

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In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Yi-Cheng TsaiProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Hiroyuki UechiFrontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan.
Sean R MillarProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Eileigh KadijkProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Alon MinkovichProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
John Paul Tsu OuyangDepartment of Biochemistry & Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA; RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Karl J SchreiberProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Jie Qi HuangProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Carina A LyonsProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Vesal KasmaeifarDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Zhixin Zhang4+4 Medical Doctor Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Rico BarsacchiMax Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
Claudia MöbiusMax Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
Antje JanoschMax Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
Jonathan C SavageMax Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
Olivia S RisslandDepartment of Biochemistry & Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA; RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Hannes RöstDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Anthony A HymanMax Planck Institute of Molecular Cell Biology and Genetics, 01307, Dresden, Germany.
Ji-Young YounProgram in Molecular Medicine, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.ORCID 0000-0001-6799-5709

Funding

Exploring the connections between translation and mRNA decayR35GM128680 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Olivia Selfridge Rissland · 2018 to 2026
$3.7M
NIGMS NIH HHS R35 GM128680
6 · The paper itself

Abstract

Cells adapt to stress by rewiring their post-transcriptional gene regulation. Stress granules-biomolecular condensates composed of polyadenylated RNAs and RNA-binding proteins-are implicated in this process, yet their precise functional roles remain debated. To address this, we mapped the dynamic proteomic landscapes of stress granules formed under oxidative and hyperosmotic stress using multi-bait BioID proximity profiling coupled with quantitative mass spectrometry. This analysis revealed context-specific remodeling of proximal interaction networks and identified a conserved, stress-dependent shift in association with the CCR4-NOT deadenylase complex. A complementary genome-wide chemical genetic screen further implicated CCR4-NOT in stress granule biology, showing that reduced CCR4-NOT activity bypassed lipoamide-mediated inhibition of stress granule assembly. Microscopy showed sequestration of the CCR4-NOT complex into stress granules, and global transcriptomic analyses revealed that this relocalization promotes poly(A) tail lengthening and increased abundance of stress-induced survival transcripts. Together, integration of proteomics, chemical genetics, and transcriptomics uncovers a spatial mechanism by which stress granule assembly promotes cellular adaptation to stress through sequestration of CCR4-NOT from the cytosol and consequent remodeling of post-transcriptional regulation.

Indexed as

CCR4-NOT complexcondensate proteomedata-independent acquisitionlipoamidepoly(A) tail regulationprotein localizationproximity-dependent biotinylationstress granules

Identifiers

PMID42094516
PMCPMC13142443

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.