Evidence map›Paper›PMID 42094392›Full record

ArticlebioRxiv : the preprint server for biology2026

Facilitating NMR Resonance Assignment with Metabolic Tampering.

Danica S Cui, Evan O Anderson, Erik Zavala, George P Lisi, J Patrick Loria

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Danica S CuiDepartment of Chemistry, Yale University, New Haven, CT 06520.
Evan O AndersonDepartment of Cellular and Molecular Physiology, Yale University, New Haven, CT 06520.
Erik ZavalaDepartment of Molecular Biophysics & Biochemistry, Yale University, New Haven, CT 06520.
George P LisiDepartment of Chemistry, Yale University, New Haven, CT 06520.
J Patrick LoriaDepartment of Chemistry, Yale University, New Haven, CT 06520.

Funding

Mechanism of catalytic regulation and active site loop motions in related protein tyrosine phosphatasesR01GM112781 · NIGMS · YALE UNIVERSITY · PI LORIA, JOSEPH P · 2015 to 2024
$2.8M
Computational and Biochemical Studies of Temperature Effects on Allostery in the Imidazole Glycerol Phosphate Synthase (IGPS) from T. maritimaR01GM106121 · NIGMS · YALE UNIVERSITY · PI BATISTA, VICTOR S, LORIA, JOSEPH P · 2014 to 2021
$2.3M
NIGMS NIH HHS R01 GM106121NIGMS NIH HHS R01 GM112781
6 · The paper itself

Abstract

The ability to assign amino acid resonances in multidimensional NMR spectra of biomolecules is necessary for detailed studies of protein structure and dynamics. Despite creative advances in isotopic labeling, unlabeling and multidimensional NMR experiments, resonance assignment remains a bottleneck in studies of large proteins. In this work, we show that the metabolic flux through biosynthetic pathways of amino acid production during protein expression can be modulated to aid in the identification of resonances in two-dimensional NMR spectra. This straightforward method involves doping

Indexed as

amino acid metabolismNMR resonance assignmentsupervised machine learningunlabeling

Identifiers

PMID42094392
PMCPMC13142442

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.