Evidence map›Paper›PMID 42094174›Full record

ArticlemedRxiv : the preprint server for health sciences2026

Optimization of head and neck cancer peripheral blood mononuclear cell processing for robust downstream functional immune analysis.

Margaret Nelson, Katelyn Jansen, Farah Sagin, Maria Lehn, Hani Alrefai, Claire Girten, Joanna Khoury, Marina Rodriguez, Jacob Garner, Carolyn Schroeder and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Margaret NelsonDivision of Hematology and Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267.
Katelyn JansenDivision of Hematology and Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267.
Farah SaginBiospecimen Shared Resource, University of Cincinnati Cancer Center, Cincinnati, OH 45267.
Maria LehnDivision of Hematology and Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267.
Hani AlrefaiDivision of Hematology and Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267.
Claire GirtenBiospecimen Shared Resource, University of Cincinnati Cancer Center, Cincinnati, OH 45267.
Joanna KhouryBiospecimen Shared Resource, University of Cincinnati Cancer Center, Cincinnati, OH 45267.
Marina RodriguezBiospecimen Shared Resource, University of Cincinnati Cancer Center, Cincinnati, OH 45267.
Jacob GarnerBiospecimen Shared Resource, University of Cincinnati Cancer Center, Cincinnati, OH 45267.
Carolyn SchroederOffice of Research, Des Moines University Medicine and Health Sciences, Des Moines, IA, USA.
Melissa MeyerDepartment of Microbiology, Immunology, and Pathology, Des Moines University Medicine and Health Sciences, Des Moines, IA, USA.
Pravin MishraOffice of Research, Des Moines University Medicine and Health Sciences, Des Moines, IA, USA.
Dalia El-GamalDivision of Hematology and Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267.
Kelsey Dillehay McKillipBiospecimen Shared Resource, University of Cincinnati Cancer Center, Cincinnati, OH 45267.
Trisha M Wise-DraperDivision of Hematology and Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, Cincinnati, OH 45267.ORCID 0000-0002-7279-4028

Funding

Optimization of peripheral blood mononuclear cell (PBMC) processing for robust downstream functional immune cell analysis and correlation with therapeutic efficacyU01CA267985 · NCI · UNIVERSITY OF CINCINNATI · PI Kelsey Dillehay McKillip, Trisha M Wise-Draper · 2022 to 2026
$1.8M
NCI NIH HHS U01 CA267985
6 · The paper itself

Abstract

The current "gold standard" for diagnosing and assessing treatment response is tumor biopsy; however, biopsies are not always feasible, safe or easily repeated during treatment. Utilization of peripheral blood mononuclear cells (PBMCs) as a surrogate for tumor biopsy allows for longitudinal sampling and is a safer, more readily available option. However, collection conditions, sample transfer time across multiple clinical sites, and PBMC processing conditions are external pre-analytical factors that must be understood and controlled to mitigate bias in downstream functional analyses. This study aims to systematically evaluate the pre-analytical variables affecting PBMC integrity and functional immune readouts as a prerequisite for downstream translational biomarker applications. Peripheral blood samples were collected from 80 treatment-naive patients with a diagnosis of head and neck squamous cell carcinoma. Blood was collected in cell preparation tubes (BD Vacutainer

Identifiers

PMID42094174
PMCPMC13142597

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.