ArticleSe pu = Chinese journal of chromatography2026
[Rapid screening and quantitative analysis of 26 soluble sugars in tobacco by ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry].
Article in Se pu = Chinese journal of chromatography, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Sugars are one of the important chemical components in tobacco. They serve as key precursor substances for various aromatic compounds in tobacco. Furthermore， they also have a significant impact on the taste and burning characteristics of tobacco. Thus， obtaining accurate information on the composition and content of soluble sugars in tobacco can provide a scientific basis for quality assessment of tobacco leaves. Moreover， it offers critical data support for quality control and process optimization. Traditional techniques for soluble sugar analysis include continuous flow analysis， ion chromatography， gas chromatography， and liquid chromatography， etc. However， these methods still have limitations such as tedious sample pretreatment， poor detection sensitivity， and low analysis efficiency. In contrast， ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry （UPLC-Q-TOF-MS） is a highly effective analytical technology. It integrates the excellent separation capability of UPLC and the superior sensitivity of MS. This combination offers great potential for qualitative and quantitative analysis of target compounds. Nonetheless， the types of soluble sugars detectable in tobacco by UPLC-Q-TOF-MS remain limited. This defect restricts the comprehensive evaluation of sugar components in tobacco. Herein， a rapid and accurate analysis method was established for high-throughput screening and quantification of 26 soluble sugars in tobacco leaves. The tobacco leaf samples were ultrasonically extracted with 30 mL 40% acetonitrile aqueous solution at 120 W for 30 min. Soluble sugar extract solution was prepared using a Waters Sep-Pak C18 solid-phase extraction cartridge. With the help of self-built sugar library， the tobacco leaf samples were screened by UPLC-Q-TOF-MS. Then matrix-matched standard calibration curves were employed to accurately quantify the target analytes. The established method was verified. The 26 soluble sugars exhibited good linear relationships with correlation coefficients （
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