Evidence map›Paper›PMID 42093082›Full record

ArticlePlant physiology2026

Overexpression of β-carboxysomes increases photosynthesis and growth in Synechocystis sp. PCC 6803.

Jinlu Hu, Kuo Zhao, Yu Chen, Xingwu Ge, Jing Yang, Gregory F Dykes, Jian-Yu Shi, Lu-Ning Liu

Abstract read
In one paragraph

Article in Plant physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Focus on algae and aquatic plants.Plant physiology · 2026
    Article
  2. Functional and structural insights into cyanobacterial COThe Plant journal : for cell and molecular biology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jinlu HuSchool of Life Science and Technology, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.ORCID 0000-0001-6125-0888
Kuo ZhaoInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.ORCID 0000-0001-9347-8254
Yu ChenInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.ORCID 0009-0009-8190-3503
Xingwu GeInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.ORCID 0009-0009-9499-8978
Jing YangInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.ORCID 0009-0009-7087-6247
Gregory F DykesInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.ORCID 0000-0002-0626-9487
Jian-Yu ShiSchool of Life Science and Technology, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.ORCID 0000-0002-2303-273X
Lu-Ning LiuInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 7ZB, United Kingdom.ORCID 0000-0002-8884-4819

Funding

Biotechnology and Biological Sciences Research Council BB/V009729/1Biotechnology and Biological Sciences Research Council BB/Y008308/1Biotechnology and Biological Sciences Research Council BB/Y01135X/1Biotechnology and Biological Sciences Research Council UKRI4524National Key R&D Program of China 2021YFA0909600National Key R&D Program of China 2023YFA0914600National Natural Science Foundation of China 32270128National Scholarship Foundation of China 202406290101Natural Environment Research Council NE/Z00019X/1
6 · The paper itself

Abstract

Cyanobacteria are photoautotrophic cell factories capable of converting carbon dioxide into valuable chemicals. The rate-limiting step in photosynthesis is the enzymatic carbon fixation catalyzed by Rubisco. The carboxysome is a protein-based organelle that plays a central role in cyanobacterial carbon fixation. It consists of thousands of subunits, including hexameric and pentameric proteins, which form a shell to encapsulate the enzymes Rubisco and carbonic anhydrase. Here, we enhanced β-carboxysome biogenesis in Synechocystis sp. PCC 6803 by overexpressing a full or partial set of endogenous β-carboxysome components. Overexpression of carboxysome proteins altered protein stoichiometry, morphology, and cellular abundance of β-carboxysomes, as well as improved photosynthetic activities and cell growth. These findings indicate that targeted carboxysome overexpression enhances photosynthetic efficiency and growth of cyanobacteria under favorable conditions. This study provides a framework for engineering cyanobacterial chassis cells as microbial cell factories and carboxysome-based CO2-concentrating mechanisms in heterologous hosts, including crop plants, to improve photosynthetic productivity for sustainable bioproduction.

Indexed as

OrganellesPhotosynthesisSynechocystisBacterial ProteinsCarbon DioxideCarbonic AnhydrasesRibulose-Bisphosphate CarboxylaseBacterial ProteinsCarbon DioxideCarbonic AnhydrasesRibulose-Bisphosphate Carboxylase

Identifiers

PMID42093082
PMCPMC13332109

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.