Evidence map›Paper›PMID 42092083›Full record

ArticleScientific reports2026

Targeting the D93 cryptic collagen epitope alters integrin α2β1-dependent cellular migration and collagen remodeling in metastatic breast cancer.

Jordan N Miner, Christopher L Emmerling, Joshua D Hamilton, Joseph Raite, Zoe Vittum, Peter C Brooks, Andre Khalil, Karissa Tilbury

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Jordan N MinerDepartment of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA.
Christopher L EmmerlingDepartment of Chemistry, University of Maine, Orono, ME, USA.
Joshua D HamiltonDepartment of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA.
Joseph RaiteDepartment of Chemistry, University of Maine, Orono, ME, USA.
Zoe VittumDepartment of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA.
Peter C BrooksGraduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME, USA.
Andre KhalilDepartment of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA.
Karissa TilburyDepartment of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA. karissa.tilbury@maine.edu.

Funding

Agilent Technologies 4845NIH HHS 1P20GM144265-01A1
6 · The paper itself

Abstract

Targeting the tumor-associated extracellular matrix (ECM) offers a promising strategy for breast cancer therapy. During cancer progression, collagen remodeling within the ECM exposes cryptic collagen epitope sites that antibodies can selectively recognize. Here, we investigate the therapeutic potential of targeting the D93 cryptic collagen epitope in 3D human metastatic breast cancer spheroids derived from MDA-MB-231 and MCF10CA1a (M4) cell lines embedded in collagen type I hydrogels. Treatment with monoclonal antibody (mAb) D93 reduced cellular migration into collagen type I hydrogels, an effect likely mediated by integrin α2β1. Two-photon microscopy further revealed that breast cancer cells drive the exposure of D93 sites and alter collagen architecture at both the fiber and fibril levels. Interestingly, collagen remodeling was altered more in the MDA-MB-231 spheroid models whereas the reduction in cellular migration was more pronounced in the M4 spheroid models, indicating a cell-line specific response to mAb D93. Together, these findings suggest that mAb D93 may inhibit integrin α2β1-dependent metastatic migration in breast cancer.

Indexed as

Breast NeoplasmsCell MovementCollagenEpitopesIntegrin alpha2beta1Antibodies, MonoclonalCell Line, TumorExtracellular MatrixFemaleHumansMDA-MB-231 CellsNeoplasm MetastasisSpheroids, CellularAntibodies, MonoclonalCollagenEpitopesIntegrin alpha2beta1Breast cancerCollagenIntegrinMigrationSecond harmonic generation (SHG) microscopySpheroids

Identifiers

PMID42092083
PMCPMC13333870

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.