Evidence map›Paper›PMID 42091808›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Multicolor Super-resolution Fluorescence Imaging of Cells by Expansion Microscopy.

Danush Taban, Marvin Jungblut, Patrick Eiring, Markus Sauer

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Danush TabanDepartment of Biotechnology & Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
Marvin JungblutRudolf Virchow Center, Research Center for Integrative and Translational Bioimaging University of Würzburg, Würzburg, Germany.
Patrick EiringDepartment of Biotechnology & Biophysics, Biocenter, University of Würzburg, Würzburg, Germany.
Markus SauerDepartment of Biotechnology & Biophysics, Biocenter, University of Würzburg, Würzburg, Germany. m.sauer@uni-wuerzburg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter presents a step-by-step protocol to resolve the organization of the nuclear pore complex (NPC) in HeLa-NUP107-GFP cells by eight-fold expansion microscopy (ExM). To enhance the efficiency of the procedure, we introduce an accelerated expansion protocol designed to allow rapid iteration and improve experimental throughput. This protocol involves both pre-expansion staining with wheat germ agglutinin (WGA) and post-digestion staining with nanobodies against GFP to visualize the NPC. The subsequent use of confocal microscopy enables multicolor super-resolution imaging with approximately 30 nm spatial resolution.

Indexed as

Nuclear PoreOptical ImagingGreen Fluorescent ProteinsHeLa CellsHumansMicroscopy, ConfocalMicroscopy, FluorescenceNuclear Pore Complex ProteinsStaining and LabelingWheat Germ AgglutininsGreen Fluorescent ProteinsNuclear Pore Complex ProteinsWheat Germ AgglutininsExpansion microscopyMulticolor fluorescence imagingNuclear pore complexSuper-resolution microscopy

Identifiers

PMID42091808

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.