ArticlePlant physiology2026
Pre-mRNA splicing regulates cellular dedifferentiation via lipid metabolism in a cytokinin-dependent manner in Arabidopsis.
Article in Plant physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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11 authors.
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Abstract
Plant cells exhibit high plasticity for proliferation and differentiation, and pre-mRNA splicing makes an important contribution to this plasticity. Here, we show that the gene responsible for root redifferentiation defective4-1 (rrd4-1), a temperature-sensitive Arabidopsis thaliana mutant with defects in adventitious rooting and callus formation from hypocotyls, encodes a homolog of yeast Ntr1, which is involved in pre-mRNA splicing. Defective callus formation in rrd4-1 at the restrictive temperature of 28 °C depended on the presence of kinetin (a synthetic cytokinin) in the callus-inducing medium. RNA-seq analysis revealed that genes involved in secondary cell wall biogenesis were upregulated, whereas those involved in cell cycle progression were downregulated, in a cytokinin-dependent manner in rrd4-1 at 28 °C. Moreover, kinetin and the rrd4-1 mutation had broad effects on alternative splicing, particularly on lipid metabolism genes such as PTPLA, SPHK2, and ATNCER1. Consistent with this result, levels of very-long-chain fatty acid (VLCFA)-type sphingolipids were reduced in rrd4-1, and kinetin affected their contents during callus induction. Kinetin enhanced the inhibitory effect of the lipid synthesis inhibitor cerulenin on callus formation, and rrd4-1 was hypersensitive to cerulenin. Together, our data suggest that pre-mRNA splicing regulates cytokinin-mediated cellular dedifferentiation through the regulation of lipid metabolism gene splicing.
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