Evidence map›Paper›PMID 42087458›Full record

ArticleJournal of fish diseases2026

Development of a Colourimetric Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Streptococcus dysgalactiae.

Tien-Tien Vicky Lau, Suat-Moi Puah, Kek-Heng Chua

Abstract read
In one paragraph

Article in Journal of fish diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Tien-Tien Vicky LauDepartment of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.ORCID https://orcid.org/0000-0002-1197-1887
Suat-Moi PuahDepartment of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.ORCID https://orcid.org/0000-0003-1346-3787
Kek-Heng ChuaDepartment of Biomedical Science, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia.ORCID https://orcid.org/0000-0002-0452-9994

Funding

Tabung Makmal Molekul Biologi & Mikrobiologi
6 · The paper itself

Abstract

Streptococcus dysgalactiae is an emerging fish pathogen that causes streptococcosis, resulting in significant economic losses in aquaculture. Conventional detection methods, such as polymerase chain reaction assays, rely on sophisticated, expensive instrumentation, which limits their applicability in resource-limited or field settings. In this study, we developed a rapid, user-friendly and closed-tube colourimetric loop-mediated isothermal amplification (LAMP) assay for the detection of S. dysgalactiae. Six primers targeting the sagB gene were designed and assay conditions were optimised to enable visual detection. The LAMP assay produced results within 50 min and detected target bacteria at concentrations as low as 500 pg (1.195 × 10

Indexed as

ColorimetryFish DiseasesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesStreptococcal InfectionsStreptococcusAnimalsDNA PrimersFishesRapid Diagnostic TestsSensitivity and SpecificityDNA Primerscolourimetricfish pathogenloop‐mediated isothermal amplificationrapidStreptococcus dysgalactiae

Identifiers

PMID42087458
PMCPMC13532613

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.