Evidence map›Paper›PMID 42086922›Full record

ArticleAnalytical and bioanalytical chemistry2026

Online Raman spectroscopy-guided strategy for efficient production of DNA recombinase UvsX in Escherichia coli.

Shaodong Liu, Junxiong Yu, Dongmei Huang, Yasong Wu, Yunlong Yang, Wenpei Lu, Juanjuan Long, Lin Wang, Yinlan Ruan, Zejian Wang and 1 more

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shaodong Liu *State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China.
Junxiong Yu *State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China.
Dongmei HuangSubphotonic Technologies Co., Ltd., Guangming District, Shenzhen, 518083, Guangdong, China.
Yasong WuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China.
Yunlong YangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China.
Wenpei LuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China.
Juanjuan LongShanghai Aimsn Medical Technology Development Co., Ltd., Shanghai, China.
Lin WangShanghai Aimsn Medical Technology Development Co., Ltd., Shanghai, China.
Yinlan RuanSchool of Optoelectronic Engineering, Guilin University of Electronic Technology, Jinji Rd., Guilin, 541004, Guangxi, China. yinlan.ruan@guet.edu.cn.
Zejian WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China. wangzejian@ecust.edu.cn.
Jiangchao QianState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China. jiangchaoqian@ecust.edu.cn.

Funding

2025 Shanghai Key Technology R&D Program for Synthetic Biology 23J219001002025 Shanghai Key Technology R&D Program for Synthetic Biology 25HC2820400National Science and Technology Major Project of China 2025ZD1203400
6 · The paper itself

Abstract

The rapid development of recombinase polymerase amplification (RPA) has driven a growing demand for UvsX, the key DNA recombinase for this technique. During bioreactor cultivation for UvsX production in Escherichia coli, biomass and substrate are critical factors affecting its efficient expression. However, the real-time monitoring techniques for these parameters remain immature, leading to delayed process control, hindering stable high-level production and precise process optimization. Using an online Raman spectrometer, this study developed synchronous quantitative prediction models for critical process parameters by partial least squares (PLS). Robust multivariate regression models showed strong correlations between predicted and measured values for biomass (R

Indexed as

Escherichia coliRecombinasesSpectrum Analysis, RamanBiomassBioreactorsFermentationLeast-Squares AnalysisRecombinasesDNA recombinase UvsXEscherichia coliOnline detectionPartial least squaresRaman spectroscopy

Identifiers

PMID42086922

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.