Evidence map›Paper›PMID 42086690›Full record

ArticleScientific reports2026

Cloning, purification and possible use of a Bacillus nitroreductase in biotechnological applications.

Firdevs Rozan Tuşar Demir, Kemal Güven, Fatma Matpan Bekler, Reyhan Gül Güven, Pınar Küce Çevik, Mustafa Abdullah Yilmaz, Seçil Yalaz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Firdevs Rozan Tuşar DemirScience Faculty, Molecular Biology and Genetics Department, Dicle University, 21280, Diyarbakir, Turkey.
Kemal GüvenScience Faculty, Molecular Biology and Genetics Department, Dicle University, 21280, Diyarbakir, Turkey. kemalg@dicle.edu.tr.
Fatma Matpan BeklerScience Faculty, Molecular Biology and Genetics Department, Dicle University, 21280, Diyarbakir, Turkey.
Reyhan Gül GüvenZiya Gökalp Education Faculty, Science and Maths Education Department, Dicle University, Diyarbakir, 21280, Turkey.
Pınar Küce ÇevikArts and Sciences Faculty, Molecular Biology and Genetics Department, Harran University, 63290, Sanlıurfa, Turkey.
Mustafa Abdullah YilmazPharmacy Faculty, Analytical Chemistry Department, Dicle University, 21280, Diyarbakır, Turkey.
Seçil YalazScience Faculty, Statistics Department, Dicle University, 21280, Diyarbakır, Turkey.

Funding

DÜBAP Projects FEN.23.011 and FEN.24.015.Scientific and Technological Research Council of Turkey (TUBITAK) the Grant Number 123Z679TUBITAK Scientist Support Programs Directorate (BIDEB) General Domestic PhD Scholarship Program 2211-A
6 · The paper itself

Abstract

Nitroreductases (NRs) can reduce nitroaromatic compounds, which are toxic, mutagenic or carcinogenic, to nitrite, amino group or hydroxylamine groups. We have utilised a cloned nitroreductase from a moderate thermophilic Bacillus species in E. coli BL21. Purification of the enzyme, followed by electrophoresis and western blotting, revealed approximate molecular weight of 30 kDa. The optimum temperature and pH of the enzyme was found as 40 °C and 8.0, respectively. Conversion of CB1954 to the metabolic products and nitrofurazone reduction by enzyme in the presence NADPH as co-factor was studied. Km, kcat and kcat/Km values were determined as 42.5 (µM), 5.07 (s

Indexed as

BacillusBacterial ProteinsBiotechnologyNitroreductasesAziridinesCloning, MolecularEscherichia coliHydrogen-Ion ConcentrationKineticsNADPNitrofurazoneSubstrate SpecificityTemperatureAziridinesBacterial ProteinsNADPNitrofurazoneNitroreductasestretazicarBacterial nitroreductaseCloningKineticsProdrug CB1954TNT biotransformation

Identifiers

PMID42086690
PMCPMC13333857

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.