Evidence map›Paper›PMID 42086564›Full record

ArticleNature communications2026

Cross-species comparison of amniote single-cell transcriptomes reveals evolutionary conservation and divergence in the chicken immune system.

Fei Wang, Jilong Ren, Yaqi Zhou, Wuqiang Huo, Huichao Liu, Mumu Qin, Yi Jing, Zhongtao Yin, Tao Shi, Huiquan Shan and 17 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

27 authors.

Fei Wang *Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.ORCID http://orcid.org/0000-0001-5998-3900
Jilong Ren *Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Yaqi Zhou *Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Wuqiang Huo *Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Huichao Liu *College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Mumu Qin *BGI Research, Sanya, China.ORCID http://orcid.org/0009-0008-9940-6225
Yi Jing *BGI Research, Sanya, China.
Zhongtao YinCollege of Animal Science and Technology, China Agricultural University, Beijing, China.ORCID http://orcid.org/0000-0002-2692-3554
Tao ShiKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Huiquan ShanKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Guanghui TanKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Haoping WangKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Xi GuoKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Junpu MeiBGI Research, Sanya, China.
Zhen YueBGI Research, Sanya, China.ORCID http://orcid.org/0000-0001-6993-6067
Zhuocheng HouCollege of Animal Science and Technology, China Agricultural University, Beijing, China.ORCID http://orcid.org/0000-0003-4752-2255
Huaijun ZhouDepartment of Animal Science, University of California, Davis, CA, USA.ORCID http://orcid.org/0000-0001-6023-9521
Jiuzhou SongDepartment of Animal and Avian Sciences, University of Maryland, College Park, MD, USA.
Søren Egedal DegnDepartment of Biomedicine, Aarhus University, Aarhus, Denmark.
Lin LinDepartment of Biomedicine, Aarhus University, Aarhus, Denmark.ORCID http://orcid.org/0000-0002-7546-4948
Yonglun LuoDepartment of Biomedicine, Aarhus University, Aarhus, Denmark.ORCID http://orcid.org/0000-0002-0007-7759
Feilong MengKey Laboratory of RNA Innovation, Science and Engineering, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0003-0333-6872
Xiaodong FangBGI Research, Sanya, China. fangxd@bgi.com.ORCID http://orcid.org/0000-0001-7061-3337
Haihan ZhangCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, China. zhhous@163.com.ORCID http://orcid.org/0000-0003-3577-8368
Xi HeCollege of Animal Science and Technology, Hunan Agricultural University, Changsha, China. hexi@hunau.edu.cn.
Lingzhao FangCenter for Quantitative Genetics and Genomics, Aarhus University, Aarhus, Denmark. lingzhao.fang@qgg.au.dk.ORCID http://orcid.org/0000-0003-1103-3679
Yu JiangKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China. yu.jiang@nwafu.edu.cn.ORCID http://orcid.org/0000-0003-4821-3585

Funding

Agriculture Research System of China (China's Agricultural Research System) CARS-41-Z08National Natural Science Foundation of China (National Science Foundation of China) 32302733
6 · The paper itself

Abstract

The molecular characterization of chicken cells provides insight into avian physiology and vertebrate evolution. Here we present a single-cell RNA-sequencing (scRNA-seq) atlas of the chicken, encompassing 1.57 million cells across 157 cell types from 36 tissues from 16 chickens, as well as spatial transcriptomes from 1 chicken embryo. We further acquire scRNA-seq data from 3 turtles (230 thousand cells, 14 tissues) and 2 ducks (22 thousand cells, 3 tissues), and integrated our data with publicly available human scRNA-seq data (960 thousand cells, 32 tissues) to explore their evolutionary conservation, particularly of immune cells. In chicken, follicular dendritic cells, unlike in mammals, exhibit myeloid rather than stromal origins and help promote B cell proliferation and migration in the bursa of Fabricius. γδ T cell subsets vary across species, reflecting the evolution of pathogen recognition and signaling mechanisms among amniotes. Our transcriptomics data thus provide a resource to study chicken cell biology and amniote evolution, highlighting immune cell similarities and distinctions arising from evolutionary divergence.

Indexed as

ChickensImmune SystemTranscriptomeAnimalsBiological EvolutionB-LymphocytesBursa of FabriciusChick EmbryoDendritic CellsEvolution, MolecularHumansSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisSpecies SpecificityTurtles

Identifiers

PMID42086564
PMCPMC13346766

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.