Evidence map›Paper›PMID 42085186›Full record

ArticleSTAR protocols2026

Protocol for isolating and culturing human monocytes from peripheral blood and measuring intracellular calcium dynamics using Fura-2.

Maria F Cano-Abad

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Maria F Cano-AbadDepartment of Pharmacology, School of Medicine, Universidad Autónoma de Madrid, Calle Arzobispo Morcillo, 4, 28029 Madrid, Spain; Instituto de Investigación Sanitaria Del Hospital Universitario de La Princesa, (IIS-IP), C/ Diego de León, 62, 28006 Madrid, Spain. Electronic address: maria.cano@uam.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for isolating and culturing human monocytes from peripheral blood and measuring intracellular calcium ([Ca

Indexed as

CalciumCell Culture TechniquesCell SeparationFura-2MonocytesCalcium SignalingCells, CulturedFlow CytometryHumansLeukocytes, MononuclearCalciumFura-2Health sciencesMolecular biologyMolecular/chemical probes

Identifiers

PMID42085186
PMCPMC13157044

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.