Intestinal Microbiomics and Liver Metabolomics Insights into the Improvement Effects of Lactiplantibacillus plantarum FJG2627 on Lipopolysaccharide-Induced Acute Liver Injury in Mice.

Endotoxin is one of the chemical substances that cause acute liver injury (ALI), which become a common health problem worldwide. Lactic acid bacteria (LAB) have emerged as a promising candidate for attenuating endotoxin-induced ALI. Lactiplantibacillus plantarum FJG2627 is a LAB isolated from traditional pickles with excellent probiotic effects. In this study, the protective functions of L. plantarum FJG2627 against ALI caused by lipopolysaccharides (LPS) in mice were investigated. Administration of L. plantarum FJG2627 significantly reduced serum ALT, AST, and pro-inflammatory factors (IL-1β and IL-6), while markedly increased the anti-inflammatory factor (IL-10). Additionally, administration of L. plantarum FJG2627 increased the abundance of beneficial bacteria such as Adlercreutzia, and reduced that of pathogenic bacteria such as Bilophila. Non-targeted metabolomics revealed that L. plantarum FJG2627 intervention altered metabolic pathways related to glucose (including galactose metabolism, etc.) and amino acid metabolisms (including histidine metabolism, etc.) in ALI mice. In summary, L. plantarum FJG2627 exerts a significant protective effect on LPS-induced ALI by controlling oxidation, suppressing inflammatory mediators, modulating intestinal microbial communities, and liver metabolites. L. plantarum FJG2627 has the potential to be developed as a food-grade Lactobacillus for safeguarding against liver damage.