ArticleJournal of fish biology2026
High-throughput multispecies quantitative polymerase chain reaction assays to study the effects of acute thermal stress in three species of Acipenser sturgeon.
Article in Journal of fish biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Fishes experience thermal stress in their natural environment that can be caused by natural or anthropogenic factors and can directly affect their physiology. In this study, we developed a multispecies OpenArray™ qPCR (quantitative polymerase chain reaction) 'chip' to measure the effects of acute thermal stress on the mRNA response of Acipenser sturgeons. The qPCR chips were then tested on three species: Acipenser fulvescens, Acipenser oxyrinchus and Acipenser brevirostrum. The A. fulvescens were from two genetically distinct populations (Burntwood River and Winnipeg River, Manitoba, Canada) that were acclimated to three different temperatures (16, 20 and 24°C) to quantify the effects of genetic background and acclimation temperatures on the transcriptomic response to acute thermal stress. Additionally, juvenile A. oxyrinchus oxyrinchus and A. brevirostrum were acclimated to 10°C and then experienced acute thermal stress. All three species were sampled for gill and liver before and after experiencing acute thermal stress, which was applied using a CT
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