ArticleNature communications2026
Improving tolerance to fluctuating light through adaptive laboratory evolution in the cyanobacterium Synechocystis.
Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Effects of the Pattern of Light and a Dark Recovery Period on Photosynthesis and Growth inMicroorganisms · 2026Article
- CurT dosage quantitatively affects thylakoid structure and PSII performance in Synechocystis sp. PCC 6803.The Plant journal : for cell and molecular biology · 2026Article
- Adaptive Laboratory Evolution inMicroorganisms · 2026Review
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Authors and funding
8 authors.
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Abstract
Fluctuating light (FL) poses a challenge to cyanobacteria by disrupting photosynthesis and damaging photosystems. Although key FL tolerance components are known, their genetic enhancement remains unexplored. We evolve Synechocystis PCC 6803 under two FL regimes (one lethal to the starter strain, LT) in order to identify previously unknown adaptive alleles. Our analysis reveals 44 mutations, 28 of which impact proteins/RNAs. Mutations in Pam68 (PSII assembly) and Sll0518, present in all strains, enhance non-lethal FL tolerance in LT. Mutated Pam68 increased PSII abundance and activity. A gain-of-function mutation in RpaB (regulator of phycobilisome association B) significantly increases tolerance to both lethal FL and high-light conditions. This is associated with an increased PSI/PSII ratio and downregulation of light harvesting. In summary, our results suggest that adaptive laboratory evolution can simultaneously identify FL tolerance factors and their advantageous alleles. The identified point mutations rewire multiple protective responses by as yet unknown molecular mechanisms.
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