Evidence map›Paper›PMID 42080366›Full record

ArticleACS nano2026

Endolysosomal Impact of Elevated Ceramide Levels Revealed by Optical and Ultrastructural Nanoprobing.

Yiqing Feng, Florian Gärber, Essa M Saied, Harshita Singh, Cecilia Spedalieri, Stephan Werner, Christoph Pratsch, Christoph Arenz, Stephan Seifert, Janina Kneipp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS nano, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yiqing FengDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Florian GärberHamburg School of Food Science, Department of Chemistry, Universität Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Essa M SaiedDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Harshita SinghDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Cecilia SpedalieriDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.ORCID 0000-0001-7480-0404
Stephan WernerDepartment X-ray Microscopy, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin, Germany.
Christoph PratschDepartment X-ray Microscopy, Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Albert-Einstein-Str. 15, 12489 Berlin, Germany.ORCID 0000-0003-4315-4854
Christoph ArenzDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.
Stephan SeifertHamburg School of Food Science, Department of Chemistry, Universität Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Janina KneippDepartment of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany.ORCID 0000-0001-8542-6331

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ceramide is a central bioactive lipid that acts as both a membrane structural component and a crucial signaling molecule in the cells. In order to maintain cellular homeostasis, ceramide metabolism is tightly regulated by enzymes in the endolysosomal system such as acid ceramidase (AC) and acid sphingomyelinase (ASM). We investigate the biochemical consequence of ceramide accumulation within the endolysosomes of living animal cells by optical nanoprobing, using surface-enhanced Raman scattering (SERS) with gold nanoparticles. The ceramide level in 3T3 fibroblast cells was systematically increased by interfering with two key enzymatic pathways in sphingolipid metabolism as well as by adding exogenous ceramide. The modulation of enzyme activity occurred by the inhibition of AC using the inhibitor N-[(2

Indexed as

CeramidesEndosomesLysosomesMetal Nanoparticles3T3 CellsAcid CeramidaseAnimalsGoldMiceSpectrum Analysis, RamanSphingomyelin PhosphodiesteraseAcid CeramidaseCeramidesGoldSphingomyelin Phosphodiesteraseacid sphingomyelinase (ASM)ceramidegold nanoparticlesN-[(2S,3R)-1,3-dihydroxyoctadecan-2-yl]2-chloroacetamide (SACLAC)random forestsurface-enhanced Raman scattering (SERS)surrogate minimal depth (SMD)

Identifiers

PMID42080366
PMCPMC13192315

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.