ArticleNucleic acids research2026
A kinetic approach for mapping seed regions of CRISPR ribonucleoprotein and improving specificity.
Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The binding of an activator (target nucleic acid) to a crRNA-Cas ribonucleoprotein (RNP) in CRISPR systems is critical to the activation, kinetics, and specificity of the CRISPR technology. Key to this activation process is the interaction between the protospacer region of the activator and the spacer region of the crRNA in the RNP complex. However, how the nucleotides in the spacer region of the crRNA contribute to the kinetics of RNP binding is not well characterized. We report here profiling of the kinetically critical regions in the process of RNP binding to activators (RNA targets). We introduced the concept and strategy of kinetic manipulators, which enabled mapping of the seed regions (6-9 nucleotides within the spacer that is sensitive to mismatches) of the CRISPR-Cas13a system, including the LbuCas13a and LwaCas13a homologs. The characterization of the binding kinetics and the introduction of kinetic manipulators provided the foundation for a new kinetic approach to improve the specificity of CRISPR techniques without sacrificing the activity. Profiling the kinetically critical regions in the CRISPR system and designing corresponding manipulators maximized the kinetic differences, between the on-target and off-target, and increased discrimination of single-nucleotide mismatches.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.