Evidence map›Paper›PMID 42080110›Full record

ArticleAvicenna journal of medical biotechnology2026

Step-by-Step Preparation of Immobilized Recombinant

Mahboobeh Nazari, Zahra Salimzadeh, Amir-Hassan Zarnani, Roya Ghods, Ramin Ghahremanzadeh

Abstract read
In one paragraph

Article in Avicenna journal of medical biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mahboobeh NazariMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Zahra SalimzadehMonoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Amir-Hassan ZarnaniDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Roya GhodsDepartment of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Ramin GhahremanzadehNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: This study highlights the significance of using Methods: The gene encoding Protein A was isolated from Results: The recombinant expression of protein A produced a yield of 50 Conclusion: The immobilization on magnetic nanoparticles presents a versatile alternative, offering the advantages of rapid separation, high surface area, and ease of handling. Magnetic nanoparticles facilitate automation and reduce processing time, making them particularly attractive for clinical and industrial applications. These immobilized forms were used to efficiently purify serum IgG, demonstrating the potential of Protein A as an effective tool for antibody isolation in biotechnological applications.

Indexed as

Enzyme-linked immuno sorbent assay (ELISA)Magnetite nanoparticlesRecombinant proteinsStaphylococcus aureus protein A (SpA)

Identifiers

PMID42080110
PMCPMC13134673

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.