Evidence map›Paper›PMID 42079050›Full record

ArticlebioRxiv : the preprint server for biology2026

AutoRNAseq: Automated Bulk RNA-seq Analysis Pipeline.

Josh Loecker, Brandt Bessell, Bhanwar Lal Puniya, Tomáš Helikar

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Josh LoeckerDepartment of Biochemistry, University of Nebraska-Lincoln.ORCID 0000-0002-6388-2958
Brandt BessellDepartment of Biochemistry, University of Nebraska-Lincoln.ORCID 0000-0003-2334-8790
Bhanwar Lal PuniyaDepartment of Biochemistry, University of Nebraska-Lincoln.ORCID 0000-0001-7528-3568
Tomáš HelikarDepartment of Biochemistry, University of Nebraska-Lincoln.ORCID 0000-0003-3653-1906

Funding

Software for collaborative construction, simulation, and analysis of mechanistic computational models of biological systemsR35GM119770 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI Tomas Helikar · 2016 to 2026
$4.4M
NIGMS NIH HHS R35 GM119770
6 · The paper itself

Abstract

Improved accessibility of high-throughput RNA sequencing has increased the amount of data generated each year. This increase in data creates a need for reproducible pipelines that can process RNA-seq data consistently across experiments. AutoRNAseq addresses this need by providing a Snakemake-based workflow for bulk RNA-seq analysis by automating data retrieval, quality control, and gene quantification. Unlike existing RNA-seq workflows that require users to coordinate multiple pipelines and pre-configure reference data, AutoRNAseq provides a single, end-to-end workflow that automates data acquisition, reference preparation, quality control, alignment, and quantification with minimal user intervention. AutoRNAseq is applicable to any domain requiring consistently processed RNA-seq datasets, including bioinformatics, computational biology, and drug-response studies.

Indexed as

pipelinereproducibilityRNA-seqSnakemakeworkflow

Identifiers

PMID42079050
PMCPMC13131636

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.