Evidence map›Paper›PMID 42078533›Full record

ArticleFrontiers in microbiology2026

A rapid isothermal RPA-CRISPR/Cas12a assay for detection of

Sezayi Ozubek, Huitao Liu, Roman R Ganta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sezayi OzubekDepartment of Pathobiology and Integrative Biomedical Sciences, College of Veterinary Medicine, Bond Life Sciences Center, University of Missouri, Columbia, MO, United States.
Huitao LiuDepartment of Pathobiology and Integrative Biomedical Sciences, College of Veterinary Medicine, Bond Life Sciences Center, University of Missouri, Columbia, MO, United States.
Roman R GantaDepartment of Pathobiology and Integrative Biomedical Sciences, College of Veterinary Medicine, Bond Life Sciences Center, University of Missouri, Columbia, MO, United States.

Funding

Rocky Mountain Spotted Fever Vaccine DevelopmentR01AI152417 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI ROMAN R. GANTA · 2021 to 2026
$3.7M
NIAID NIH HHS R01 AI152417
6 · The paper itself

Abstract

Introduction: Rocky Mountain spotted fever (RMSF) resulting from the tick-borne Methods: Here we established a fast, fully isothermal RPA-CRISPR/Cas12a assay that targeted the vitamin uptake transporter ( Results: Using quantified synthetic DNA (gBlock) standards derived from the Discussion: The findings of this study support the feasibility of RPA-Cas12a as a rapid molecular workflow for

Indexed as

isothermal amplificationmolecular diagnosisrapid detectionRickettsia rickettsiiRocky Mountain spotted feverRPA–Cas12a assay

Identifiers

PMID42078533
PMCPMC13128586

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.