ArticleWellcome open research2025
LwaCas13a indiscriminately targets the human transcriptome.
Article in Wellcome open research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- LwaCas13a indiscriminately targets the human transcriptome.Wellcome open research · 2025Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein 13a (Cas13a) has been described as a superior tool to short-interfering RNAs (siRNAs) for specific gene silencing. Cas13 targets RNAs through Watson-Crick binding of the target CRISPR RNA (crRNA) and activation of nuclease activity. In bacteria, once Methods: We developed a doxycycline-inducible system to express LwaCas13a in HEK293T cells. The off-target activity of LwaCas13a in HEK293T cells was analyzed using RNA-seq. Results: In this study, we observed that activation of LwaCas13a in HEK293T cells led to non-specific targeting of RNAs, which caused cell toxicity and death. Conclusion: This study provides evidence of the off-target activity of LwaCas13a in HEK293T cells, making it an unsuitable tool for the specific downregulation of RNAs.
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