ArticleSensors (Basel, Switzerland)2026
A Wearable Electrochemical Sensing Platform for Rapid Detection of Organophosphorus Pesticides: A Flexible Biosensor Based on Screen-Printed Electrodes and Organophosphorus Hydrolase.
Article in Sensors (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The rapid detection of organophosphorus (OP) compounds is crucial for safeguarding human health and ensuring food safety. This study presents a novel wearable electrochemical biosensor that integrates miniaturized screen-printed electrodes with wearable devices to achieve real-time, on-site OP detection. The biosensor was fabricated by constructing a screen-printed carbon electrode (SPCE) on a thermoplastic polyurethane (TPU) substrate, sequentially modified with graphene (GR), gold nanoparticles (AuNPs), and organophosphorus hydrolase (OPH), and finally encapsulated with Nafion. This SPCE/GR/AuNPs/OPH/Nafion configuration yields a highly flexible and portable device. The detection principle relies on the enzymatic hydrolysis of methyl paraoxon (MPOX) by OPH, generating p-nitrophenol (PNP), which is quantitatively measured via square wave voltammetry (SWV). The sensor exhibits a broad linear detection range (30-400 μM) with a strong linear correlation (R
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