Evidence map›Paper›PMID 42075686›Full record

ArticlePathogens (Basel, Switzerland)2026

Detection of Dengue Virus Serotype 3 Using a Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay: Evaluation with Clinical Samples from Southeastern Mexico.

Perla Pérez-Tepos, Gilma Guadalupe Sánchez-Burgos, Beatriz Xoconostle-Cázares, Gloria María Molina-Salinas, Julio Huchín-Cetz, Edgar Sevilla-Reyes, Berenice Calderón-Pérez, Roberto Ruiz-Medrano, Rosalia Lira

Abstract readEvaluation Study
In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Perla Pérez-TeposUnidad de Investigación Biomédica Oncológica Genómica, Hospital Gineco Pediatría 3A, OOAD Cd Mx Norte, Instituto Mexicano del Seguro Social, Ciudad de Mexico 07760, Mexico.ORCID 0009-0008-2334-9447
Gilma Guadalupe Sánchez-BurgosUnidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida 97150, Yucatán, Mexico.ORCID 0000-0002-7112-1489
Beatriz Xoconostle-CázaresDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de Mexico 07360, Mexico.ORCID 0000-0002-7366-7725
Gloria María Molina-SalinasUnidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida 97150, Yucatán, Mexico.ORCID 0000-0002-3961-6449
Julio Huchín-CetzLaboratorio de Apoyo a la Vigilancia Epidemiológica, Unidad de Investigación Médica Yucatán, Instituto Mexicano del Seguro Social, Mérida 97150, Yucatán, Mexico.
Edgar Sevilla-ReyesLaboratorio de Transcriptómica e Inmunología Molecular, Instituto Nacional de Enfermedades Respiratorias "Ismael Cosío Villegas", Ciudad de Mexico 14080, Mexico.ORCID 0000-0002-7047-0222
Berenice Calderón-PérezDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de Mexico 07360, Mexico.ORCID 0000-0002-6144-1411
Roberto Ruiz-MedranoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de Mexico 07360, Mexico.ORCID 0000-0003-0223-7776
Rosalia LiraUnidad de Investigación Biomédica Oncológica Genómica, Hospital Gineco Pediatría 3A, OOAD Cd Mx Norte, Instituto Mexicano del Seguro Social, Ciudad de Mexico 07760, Mexico.ORCID 0000-0002-6812-9471

Funding

Secretaría de Ciencia Tecnología e Innovación CBF-2023-2024-4199Secretaría de Ciencia Tecnología e Innovación CBF-2025-I-3383
6 · The paper itself

Abstract

Dengue virus (DENV), an important mosquito-borne orthoflavivirus, represents a growing global threat due to its geographic expansion and recent outbreaks worldwide. In resource-limited endemic settings, the development of affordable diagnostic assays is needed. In this study, we developed and validated a colorimetric reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for the detection of DENV type 3 (DENV-3) using 95 previously diagnosed clinical samples from Southeastern Mexico. Primers targeting the 3' untranslated region (3' UTR) of DENV-3 were designed, and assay conditions were standardized. The colorimetric RT-LAMP DENV-3 system achieved a preliminary limit of detection of 1 × 10

Indexed as

ColorimetryDengueDengue VirusMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesDNA PrimersHumansMexicoRapid Diagnostic TestsReverse TranscriptionRNA, ViralSensitivity and SpecificitySerogroupDNA PrimersRNA, Viralcolorimetric assaydengue virus (DENV)RT-LAMP

Identifiers

PMID42075686
PMCPMC13118920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.