Evidence map›Paper›PMID 42074522›Full record

ArticleGenes2026

Genome Integrity in Dairy Cows Fed Black Soldier Fly Oil: An Integrated Sister Chromatid Exchange and Alkaline Comet In Vivo Assessment.

Alfredo Pauciullo, Giustino Gaspa, Viviana Genualdo, Cristina Rossetti, Angela Perucatti, Giulia Milanese, Martina Alessandra Gini, Flavia Caserta, Lara Rastello, Mathieu Gerbelle and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Alfredo PauciulloDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0002-3140-9373
Giustino GaspaDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0003-1604-8917
Viviana GenualdoInstitute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy, Piazzale Enrico Fermi 1, 80055 Portici, Italy.ORCID 0000-0001-7635-4508
Cristina RossettiInstitute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy, Piazzale Enrico Fermi 1, 80055 Portici, Italy.ORCID 0000-0003-1944-0068
Angela PerucattiInstitute for the Animal Production System in the Mediterranean Environment, National Research Council of Italy, Piazzale Enrico Fermi 1, 80055 Portici, Italy.
Giulia MilaneseIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Torino, Italy.ORCID 0009-0005-2000-1240
Martina Alessandra GiniIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154 Torino, Italy.ORCID 0009-0004-3110-6972
Flavia CasertaDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.
Lara RastelloDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0001-5185-2797
Mathieu GerbelleInstitut Agricole Régional, Regione La Rochère 1/A, 11100 Aosta, Italy.
Alessandro GalliFerrero Mangimi S.p.A., Via Fornace 15, 12060 Farigliano, Italy.ORCID 0009-0003-6683-1819
Laura GascoDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0002-1829-7936
Manuela RennaDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.ORCID 0000-0003-4296-7589

Funding

Italian Ministry of Agriculture, Food Sovereignty and Forests CUP: D23 C23000570005
6 · The paper itself

Abstract

BACKGROUND/

objectivesInsect-derived lipids are emerging as circular-economy feed ingredients, but their implementation in ruminant diets requires robust safety assessment beyond productive endpoints. This study evaluated genome integrity in 26 lactating Valdostana Red Pied cows fed concentrates containing either hydrogenated palm fat (HPF;

methodsPeripheral blood lymphocytes were analyzed using Sister Chromatid Exchanges (SCE), reflecting replication-associated chromosomal instability, and the alkaline Comet assay, quantifying primary DNA damage at the single-cell level (Tail DNA and Olive tail moment) at T0 (the day before the start of the two experimental diets), T1 (30 d) and T2 (50 d).

resultsBaseline SCE frequencies were comparable between groups. Over time, SCE values decreased in both groups, but a significant reduction occurred only in HIO at day 50, with lower SCE frequency than HPF (5.73 ± 0.11 vs. 6.29 ± 0.13;

conclusionsOverall, no evidence of genotoxicity was observed in cows fed HIO; conversely, the results support compatibility with genome stability and suggest a modest time-dependent improvement detectable mainly after prolonged supplementation.

Indexed as

Animal FeedSister Chromatid ExchangeAnimalsCattleComet AssayDietDNA DamageFemaleLymphocytesgenome-damaging effectsgenotoxicityHermetia illucens oilinsect derived mealruminant

Identifiers

PMID42074522
PMCPMC13116937

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.