Evidence map›Paper›PMID 42074294›Full record

ArticleInternational journal of molecular sciences2026

Multimodal Single-Cell Transcriptomic and Chromatin Accessibility Profiling Reveals Monocyte-Derived Macrophage Dynamics Following Ischemic Stroke.

Milton H Hamblin, Rabi Murad, Austin C Boese, Huijie Huang, Rebecca A Porritt, Tanvi Bobba, Jean-Pyo Lee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Milton H HamblinDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA 92521, USA.ORCID 0009-0006-8538-4841
Rabi MuradNCI-Designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA.ORCID 0009-0004-5297-8307
Austin C BoeseSchool of Medicine, Emory University, Atlanta, GA 30322, USA.
Huijie HuangCenter for Neurological Diseases, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA.ORCID 0000-0003-2471-8351
Rebecca A PorrittNCI-Designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA.
Tanvi BobbaHealth Sciences Center, Tulane University, New Orleans, LA 70112, USA.ORCID 0009-0001-7966-3186
Jean-Pyo LeeDivision of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA 92521, USA.

Funding

Combination treatment of ischemic stroke with perlecan DV and neural stem cellsR01NS110370 · NINDS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI BIX, GREGORY JAYE, LEE, JEAN-PYO · 2019 to 2023
$2.4M
NINDS NIH HHS 7R01NS110370NINDS NIH HHS R01 NS110370
6 · The paper itself

Abstract

Ischemic stroke promotes monocyte recruitment to the injured brain and their differentiation into monocyte-derived macrophages (MDMs). These cells contribute to debris clearance but may also exacerbate neuroinflammation. However, the heterogeneity of MDM subsets and the phenotypic transitions that shape MDM functional states during the subacute phase of stroke remain incompletely characterized. To address this, we first performed single-cell RNA sequencing (scRNA-seq) to define the transcriptional landscape of the mouse brain 48 h after transient middle cerebral artery occlusion/reperfusion compared with sham controls. Reclustering of macrophage-lineage cells identified multiple MDM subsets, including a distinct

Indexed as

ChromatinIschemic StrokeMacrophagesMonocytesTranscriptomeAnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticChromatin Assembly and DisassemblyGene Expression ProfilingLysosomesMaleMiceMice, Inbred C57BLSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisAntigens, CDAntigens, Differentiation, MyelomonocyticChromatinischemic strokemonocyte-derived macrophagessingle-cell chromatin profilingsingle-cell transcriptomics

Identifiers

PMID42074294
PMCPMC13115867

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.