ArticleInternational journal of molecular sciences2026
Ultrasound-Assisted Optimization of the Activation and Inactivation of Thermostable α-Amylase.
Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ultrasound is a non-thermal technology increasingly applied in food processing to modulate enzyme activity. This study investigated the effects of ultrasonic irradiation on the activity of a commercial thermostable α-amylase. Enzyme activity was determined by quantifying reducing sugars released from starch using the 3,5-dinitrosalicylic acid method, and protein concentration was measured by the Bradford assay. A one-factor-at-a-time approach was used to evaluate the effects of ultrasonic amplitude, treatment time, enzyme concentration, incubation temperature, and calcium ion concentration. Subsequently, a Box-Behnken design was applied to analyze the combined influence of amplitude, treatment duration, temperature, and calcium concentration on residual activity. The enzyme exhibited an initial activity of 46.27 ± 3.63 U/mL under standard assay conditions. Moderate ultrasonic amplitudes enhanced activity, whereas prolonged exposure and elevated temperatures promoted inactivation. Statistical analysis showed that the incubation temperature and calcium concentration significantly influenced residual activity, and the quadratic model provided a good fit (R
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