Evidence map›Paper›PMID 42074057›Full record

ArticleInternational journal of molecular sciences2026

Mixing Is Dispensable for Optical Density-Based High-Throughput Growth Screening Assay in Fission Yeast.

Kim Kiat Lim, Jiunn Jye Chung, Sha Ma, Ching-Chiuan Yen, Louxin Zhang, Ee Sin Chen

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kim Kiat LimDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.ORCID 0000-0003-0340-5903
Jiunn Jye ChungNUS Centre for Additive Manufacturing, National University of Singapore, Singapore 117575, Singapore.
Sha MaNUS Centre for Additive Manufacturing, National University of Singapore, Singapore 117575, Singapore.
Ching-Chiuan YenNUS Centre for Additive Manufacturing, National University of Singapore, Singapore 117575, Singapore.ORCID 0000-0003-4325-1689
Louxin ZhangDepartment of Mathematics, Faculty of Science, National University of Singapore, Singapore 119076, Singapore.
Ee Sin ChenDepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.ORCID 0000-0002-7147-2131

Funding

National University Health System NUHSRO/2023/028/NUSMed/12/LOANational University of Singapore NAII-SG-2025-019Singapore Ministry of Education NUHSRO/2023/043/T1/Seed-Mar/03
6 · The paper itself

Abstract

Optical density (OD)-based cell growth measurement is commonly used in high-throughput screening (HTS) during drug discovery or when deciphering the pharmaceutical mechanism of action. While resuspending the cells via a mixing step is often assumed to be necessary prior to OD measurement, its essentiality in HTS workflows has not been systematically verified. Here, through the measurement of the growth of several strains of the microbial yeast

Indexed as

High-Throughput Screening AssaysSchizosaccharomycesdoubling timeexponential phasefission yeastgrowth ratemicrotiter plateSchizosaccharomyces pombe

Identifiers

PMID42074057
PMCPMC13115688

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.