Evidence map›Paper›PMID 42073254›Full record

ArticleFoods (Basel, Switzerland)2026

Validation of a Duplex Digital PCR Assay for the Quantification of the NK603 Maize Event Across Three dPCR Platforms.

Daniela Verginelli, Katia Spinella, Sara Ciuffa, Raffaele Carrano, Davide La Rocca, Elisa Pierboni, Monica Borghi, Silvana Farneti, Ugo Marchesi

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Daniela VerginelliNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.ORCID 0000-0001-9247-7073
Katia SpinellaNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.ORCID 0000-0003-3839-7974
Sara CiuffaNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.
Raffaele CarranoNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.
Davide La RoccaNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.ORCID 0000-0001-9456-359X
Elisa PierboniIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", 06126 Perugia, Italy.ORCID 0000-0003-3488-5743
Monica BorghiIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", 06126 Perugia, Italy.ORCID 0000-0002-6337-5999
Silvana FarnetiIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", 06126 Perugia, Italy.
Ugo MarchesiNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.ORCID 0000-0002-7789-241X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the European Union, mandatory labeling of food and feed products is required when authorized genetically modified organisms (GMOs) exceed 0.9% per ingredient, necessitating reliable analytical methods for official control laboratories. Event-specific PCR assays validated according to ISO/IEC 17025 are the reference approach for GMO detection, identification, and quantification. The growing use of digital PCR (dPCR) has encouraged the adaptation of real-time PCR methods to dPCR-based strategies, as dPCR enables absolute quantification without calibration standards, shows reduced sensitivity to inhibitors, and allows for the design of a multiplex assay. In this study, an

Indexed as

digital PCRGMOvalidation study

Identifiers

PMID42073254
PMCPMC13114549

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.