Evidence map›Paper›PMID 42070236›Full record

ArticleSTAR protocols2026

Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103

Damir Vurnek, Giorgi Tchitashvili, Anna Seichter, Tomislav Vuletic, Isabel Heß, Lizi Trapaidze, Lukas Heger, Nounagnon Romaric Tochoedo, Christian H K Lehmann, Bart N Lambrecht and 2 more

Abstract read
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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Damir VurnekInstitute of Immunology, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, 91052 Erlangen, Germany. Electronic address: damir.vurnek@med.uni-jena.de.
Giorgi TchitashviliLaboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, 91052 Erlangen, Germany.
Anna SeichterLaboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, 91052 Erlangen, Germany; Department of Gastroenterology and Hepatology, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.
Tomislav VuleticInstitute of Physics, 10000 Zagreb, Croatia.
Isabel HeßVIB, Suzanne Tassierstraat 1, 9052 Gent, Belgium.
Lizi TrapaidzeInstitute of Immunology, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Lukas HegerDepartment of Transfusion Medicine and Hemostaseology, University Hospital Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg, 91054 Erlangen, Germany.
Nounagnon Romaric TochoedoInstitute of Immunology, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany.
Christian H K LehmannLaboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, 91052 Erlangen, Germany; Department of Pediatrics and Adolescent Medicine, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany; FAU Profile Center Immunomedicine (FAU I-MED), 91054 Erlangen, Germany.
Bart N LambrechtLaboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, 9052 Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium; Department of Pulmonary Medicine, Erasmus University Medical Center Rotterdam, 3015 Rotterdam, the Netherlands.
Diana DudziakInstitute of Immunology, Jena University Hospital, Friedrich-Schiller-University Jena, 07743 Jena, Germany; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, 91052 Erlangen, Germany; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, 07743 Jena, Germany; Center for Sepsis Control and Care, Jena University Hospital, 07747 Jena, Germany; Zentrum für Alternsforschung Jena (ZAJ), Jena, Germany; Comprehensive Cancer Center Central Germany (CCCG), 07743 Jena, Germany; Core Facility Cytometry, Jena University Hospital, 07743 Jena, Germany. Electronic address: diana.dudziak@med.uni-jena.de.
Lukas AmonLaboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital, Friedrich-Alexander University of Erlangen-Nürnberg, 91052 Erlangen, Germany; Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, 9052 Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium. Electronic address: lukas.amon@irc.vib-ugent.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ex vivo differentiation platforms have a long-standing history for studying dendritic cell (DC) ontogeny and function. Here, we present a protocol for differentiating bona fide DCs from murine bone marrow. We describe steps for bone marrow preparation, followed by ex vivo differentiation of DC subtypes resembling either lymphoid or mucosal tissue DC phenotypes. We then detail procedures for flow cytometric analysis and sorting strategies that facilitate phenotypic and functional studies of murine DC subtypes and/or their bone marrow progenitors. For complete details on the use and execution of this protocol, please refer to Amon et al.

Indexed as

Antigens, CDBone Marrow CellsCell DifferentiationDendritic CellsFlow CytometryMyeloid Progenitor CellsAnimalsCell SeparationIntegrin alpha ChainsMicealpha E integrinsAntigens, CDIntegrin alpha ChainsCell-based AssaysCell cultureCell DifferentiationCell isolationFlow CytometryImmunology

Identifiers

PMID42070236
PMCPMC13147993

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.