Evidence map›Paper›PMID 42069610›Full record

ArticleStem cell research & therapy2026

Generation and single-cell characterization of functional megakaryocytes derived from umbilical cord blood.

Yanmin He, Yue Liang, Ji He, Yu Shen, Zhipan Wu, Shanshan Pei, Faming Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yanmin He *Institute of Transfusion Medicine, Blood Center of Zhejiang Province, 789 JianYe Road, Hangzhou, Zhejiang, 310052, People's Republic of China.
Yue Liang *Bone Marrow Transplantation Center of The First Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Office 518, 1369 Wenyixi Road, Hangzhou, Zhejiang, 311121, People's Republic of China.
Ji HeInstitute of Transfusion Medicine, Blood Center of Zhejiang Province, 789 JianYe Road, Hangzhou, Zhejiang, 310052, People's Republic of China.
Yu ShenBone Marrow Transplantation Center of The First Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Office 518, 1369 Wenyixi Road, Hangzhou, Zhejiang, 311121, People's Republic of China.
Zhipan WuInstitute of Transfusion Medicine, Blood Center of Zhejiang Province, 789 JianYe Road, Hangzhou, Zhejiang, 310052, People's Republic of China.
Shanshan PeiBone Marrow Transplantation Center of The First Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Office 518, 1369 Wenyixi Road, Hangzhou, Zhejiang, 311121, People's Republic of China. shanshan.pei@zju.edu.cn.
Faming ZhuInstitute of Transfusion Medicine, Blood Center of Zhejiang Province, 789 JianYe Road, Hangzhou, Zhejiang, 310052, People's Republic of China. zfm00@hotmail.com.

Funding

Department of Science and Technology of Zhejiang Provinc 2023R01012Key Research and Development Program of Zhejiang Province 2022C03005National Natural Science Foundation of China 82270157Zhejiang Provincial Natural Science Foundation of China LTGY23H080002
6 · The paper itself

Abstract

backgroundGeneration of megakaryocytes (MKs) from stem cells in vitro to produce platelets (PLTs) is an appealing approach for providing an alternative source of PLTs. Understanding the transcriptomic characteristics of MKs in vitro is crucial for providing a theoretical foundation for producing functional MKs more efficiently in the future.

methodsCD34

resultsUCB-derived MKs exhibited typical characteristics of MKs in vivo, including morphology, polyploidy, and subcellular structure. The mRNA expression levels of GATA1, FOG1, NF-E2, FLI1, CD41, and CD61 on Days 10 and 14 were significantly greater than those on Day 4. CD62P expression on the surface of UCB-PLTs increased markedly in response to thrombin or TRAP6 stimulation. Humanized PLTs were also detected in the peripheral blood of NCG mice following the infusion of UCB-MKs. According to the results of the sc-RNAseq analysis, nine transcriptionally distinct clusters of UCB-MKs, labeled MK1-MK9, were identified, with only the MK9 population being related to immunity. The MK1-MK8 populations displayed typical MK characteristics and were the most prevalent subtypes. In addition, compared with hESCs, UCB-derived MKs exhibited a greater proportion of active-cycling MKs with strong differentiation potential.

conclusionsIn conclusion, this study describes the biological functions and transcriptomic profile of MKs derived from UCB, which will aid in the further development of more efficient systems for generating MKs in vitro and promote their application in cellular therapy.

Indexed as

Fetal BloodMegakaryocytesAnimalsBlood PlateletsCell DifferentiationCells, CulturedGATA1 Transcription FactorHematopoietic Stem CellsHumansMiceSingle-Cell AnalysisGATA1 protein, humanGATA1 Transcription FactorIn vitroMegakaryocytesPlateletsSingle-cell RNA sequencingUmbilical cord blood

Identifiers

PMID42069610
PMCPMC13312762

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.