Cavitation behaviors of genetically engineered bacterial protein nanoparticles induced by pulsed ultrasound.

Feng Gao, Haiyan Yang, Xiaoqing Luo, Ting Gong, Jianzhong Zou, Dui Qin, Yufeng Zhou

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Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Feng GaoState Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing 400016, China.

Haiyan YangDepartment of Ultrasound, Chongqing Academy of Medical Sciences, Chongqing General Hospital, Chongqing University, Chongqing 401147, China.

Xiaoqing LuoState Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing 400016, China.

Ting GongDepartment of Ultrasound, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 611731, China.

Jianzhong ZouState Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing 400016, China.

Dui QinDepartment of Ultrasound, Chongqing Academy of Medical Sciences, Chongqing General Hospital, Chongqing University, Chongqing 401147, China; Department of Biomedical Engineering, School of Life Health Information Science and Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

Yufeng ZhouState Key Laboratory of Ultrasound in Medicine and Engineering, Chongqing Medical University, Chongqing 400016, China. Electronic address: yufeng.zhou@cqmu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetically engineered bacterial protein nanoparticles (GVs-E.coli) enhance the therapeutic efficacy of high-intensity focused ultrasound (HIFU) by virtue of their intrinsic tumor tropism, favorable biosafety profile, and inherent cavitation activity. However, the cavitation behavior of GVs-E.coli has not been systematically characterized, and the underlying regulatory mechanisms remain poorly elucidated presenting a critical knowledge gap that limits their optimal theranostic application. Herein, we investigated the cavitation behaviors of GVs-E.coli within a wall-free flow channel embedded in tissue-mimicking agarose phantoms using a customized ultrasound platform. Passive cavitation detection (PCD) was performed under varying peak negative pressures (PNPs) and bacterial concentrations, with acoustic emissions analyzed in both time and frequency domains. Our results revealed that the stable cavitation (SC) threshold of GVs-E.coli was 1.6 MPa and the inertial cavitation (IC) threshold was 2.2 MPa. Cavitation dose was positively correlated with PNP, yet harmonics and sub-harmonics exhibited distinct growth patterns.A critical concentration threshold of OD

Indexed as

Bacterial ProteinsNanoparticlesProtein EngineeringUltrasonic WavesEscherichia coliBacterial ProteinsCavitation thresholdGenetically engineered bacterial protein nanoparticlesInertial cavitationPassive cavitation detectionStable cavitation

Identifiers

PMID42068786

DOI10.1016/j.ultsonch.2026.107874

PMCPMC13147375

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.